Four-way junction formation promoting ultrasensitive electrochemical detection of MicroRNA

Mahmoud Labib, Shahrokh M. Ghobadloo, Nasrin Khan, Dmitry M. Kolpashchikov, Maxim V. Berezovski*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (∼36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis.

Original languageEnglish (US)
Pages (from-to)9422-9427
Number of pages6
JournalAnalytical Chemistry
Volume85
Issue number20
DOIs
StatePublished - Oct 15 2013
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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