TY - JOUR
T1 - Four-way junction formation promoting ultrasensitive electrochemical detection of MicroRNA
AU - Labib, Mahmoud
AU - Ghobadloo, Shahrokh M.
AU - Khan, Nasrin
AU - Kolpashchikov, Dmitry M.
AU - Berezovski, Maxim V.
PY - 2013/10/15
Y1 - 2013/10/15
N2 - MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (∼36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis.
AB - MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (∼36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis.
UR - http://www.scopus.com/inward/record.url?scp=84886876486&partnerID=8YFLogxK
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U2 - 10.1021/ac402416z
DO - 10.1021/ac402416z
M3 - Article
C2 - 24047131
AN - SCOPUS:84886876486
SN - 0003-2700
VL - 85
SP - 9422
EP - 9427
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 20
ER -
