FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function

Yeqing A. Yang, Jonathan C. Zhao, Ka Wing Fong, Jung Kim, Shangze Li, Chunxiao Song, Bing Song, Bin Zheng, Chuan He, Jindan Yu*

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Forkhead box A1 (FOXA1) is an FKHD family protein that plays pioneering roles in lineage-specific enhancer activation and gene transcription. Through genome-wide location analyses, here we show that FOXA1 expression and occupancy are, in turn, required for the maintenance of these epigenetic signatures, namely DNA hypomethylation and histone 3 lysine 4 methylation. Mechanistically, this involves TET1, a 5-methylcytosine dioxygenase. We found that FOXA1 induces TET1 expression via direct binding to its cis-regulatory elements. Further, FOXA1 physically interacts with the TET1 protein through its CXXC domain. TET1 thus co-occupies FOXA1-dependent enhancers and mediates local DNA demethylation and concomitant histone 3 lysine 4 methylation, further potentiating FOXA1 recruitment. Consequently, FOXA1 binding events are markedly reduced following TET1 depletion. Together, our results suggest that FOXA1 is not only able to recognize but also remodel the epigenetic signatures at lineage-specific enhancers, which is mediated, at least in part, by a feed-forward regulatory loop between FOXA1 and TET1.

Original languageEnglish (US)
Pages (from-to)8153-8164
Number of pages12
JournalNucleic acids research
Volume44
Issue number17
DOIs
StatePublished - Sep 30 2016

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ASJC Scopus subject areas

  • Genetics

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