Frequency of pilin antigenic variation in Neisseria gonorrhoeae

C. D. Serkin, H. S. Seifert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Variation of the pilus of Neisseria gonorrhoeae occurs by the recombination of silent pilin DNA sequences into the pilin expression locus. We have developed a quantitative, competitive reverse transcription-PCR assay which measures the frequency of pilin antigenic variation independently of changes in gonococcal colony morphology and have determined this frequency within a gonococcal population. We have also studied the frequency of antigenic variation during growth and have concluded that growth does not dramatically influence the frequency of pilin antigenic variation, although a reproducible, twofold increase is observed upon the transition into late log/stationary phase.

Original languageEnglish (US)
Pages (from-to)1955-1958
Number of pages4
JournalJournal of bacteriology
Volume180
Issue number7
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Fingerprint

Dive into the research topics of 'Frequency of pilin antigenic variation in Neisseria gonorrhoeae'. Together they form a unique fingerprint.

Cite this