Abstract
Variation of the pilus of Neisseria gonorrhoeae occurs by the recombination of silent pilin DNA sequences into the pilin expression locus. We have developed a quantitative, competitive reverse transcription-PCR assay which measures the frequency of pilin antigenic variation independently of changes in gonococcal colony morphology and have determined this frequency within a gonococcal population. We have also studied the frequency of antigenic variation during growth and have concluded that growth does not dramatically influence the frequency of pilin antigenic variation, although a reproducible, twofold increase is observed upon the transition into late log/stationary phase.
Original language | English (US) |
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Pages (from-to) | 1955-1958 |
Number of pages | 4 |
Journal | Journal of bacteriology |
Volume | 180 |
Issue number | 7 |
DOIs | |
State | Published - 1998 |
ASJC Scopus subject areas
- Molecular Biology
- Microbiology