Abstract
Inhibitory cell surface proteins on T cells are often dynamically regulated, which contributes to their physiologic function. PECAM-1 (CD31) is an inhibitory receptor that facilitates TGF-β-mediated suppression of T cell activity. It is well established in CD4 + T cells that PECAM-1 is expressed in naïve recent thymic emigrants, but is down-regulated after acute T cell activation and absent from memory cells. The extent to which PECAM-1 expression is similarly regulated in CD8 + T cells is much less well characterized. We evaluated T cells recovered from mice after infection with a model intracellular pathogen and determined that, in CD8 + T cells, PECAM-1 expression was strongly down-regulated during acute infection but re-expressed to intermediate levels in memory cells. Down-regulation of PECAM-1 expression in CD8 + T cells was transcriptionally regulated and affected by the strength and nature of TCR signaling. PECAM-1 was also detected on the surface of human activated/memory CD8 + , but not CD4 + T cells. These data demonstrate that PECAM-1 expression is dynamically regulated, albeit differently, in both CD4 + and CD8 + T cells. Furthermore, unlike memory CD4 + T cells, memory CD8 + T cells retain PECAM-1 expression and have the potential to be modulated by this inhibitory receptor.
Original language | English (US) |
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Pages (from-to) | 883-893 |
Number of pages | 11 |
Journal | Journal of Leukocyte Biology |
Volume | 104 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2018 |
Keywords
- CD8 T cell
- Listeria monocytogenes
- PECAM-1
- diacylglycerol kinase zeta
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology