Full Factorial Microfluidic Designs and Devices for Parallelizing Human Pluripotent Stem Cell Differentiation

Duncan M. Chadly, Andrew M. Oleksijew, Kyle S. Coots, Jose J. Fernandez, Shun Kobayashi, John A. Kessler, Akihiro J. Matsuoka*

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Human pluripotent stem cells (hPSCs) are promising therapeutic tools for regenerative therapies and disease modeling. Differentiation of cultured hPSCs is influenced by both exogenous factors added to the cultures and endogenously secreted molecules. Optimization of protocols for the differentiation of hPSCs into different cell types is difficult because of the many variables that can influence cell fate. We present microfluidic devices designed to perform three- and four-factor, two-level full factorial experiments in parallel for investigating and directly optimizing hPSC differentiation. These devices feature diffusion-isolated, independent culture wells that allow for control of both exogenous and endogenous cellular signals and that allow for immunocytochemistry (ICC) and confocal microscopy in situ. These devices are fabricated by soft lithography in conjunction with 3D-printed molds and are operable with a single syringe pump, eliminating the need for specialized equipment or cleanroom facilities. Their utility was demonstrated by on-chip differentiation of hPSCs into the auditory neuron lineage. More broadly, these devices enable multiplexing for experimentation with any adherent cell type or even multiple cell types, allowing efficient investigation of the effects of medium conditions, pharmaceuticals, or other soluble reagents.

Original languageEnglish (US)
Pages (from-to)41-54
Number of pages14
JournalSLAS Technology
Volume24
Issue number1
DOIs
StatePublished - Feb 1 2019

Keywords

  • factorial design
  • inner ear
  • microfluidics
  • pluripotent stem cells
  • stem cell transplantation

ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

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