Full systems considerations for multiphoton microscopy

D. L. Wokosin; J. M. Girkin

doi:10.1117/12.424558

Full systems considerations for multiphoton microscopy

D. L. Wokosin, J. M. Girkin

Neuroscience

Research output: Contribution to journal › Article › peer-review

Abstract

A most-important-variables analysis of practical, successful multiphoton excitation fluorescence microscopy is presented. The key strength of multiphoton imaging localization of the excitation volume-helps to decouple the excitation from the emission; the emission no longer needs to be imaged. The presentation starts with maximizing the detected signal and proceeds to the laser source considerations. The main goal is to match the instrumentation to the biological problem. The main aspects covered are… Emission collection: potential signal Focal plane mismatch: chromatic aberration Light detected: actual signal Live cell imaging: preservation of biological function Deep imaging: preservation of image contrast Signal production: potential limitations.

Original language	English (US)
Pages (from-to)	231-237
Number of pages	7
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4262
DOIs	https://doi.org/10.1117/12.424558
State	Published - 2001

Keywords

Confocal
Laser scanning fluorescence microscopy
Multi-photon excitation
Objective lenses

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.424558

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title = "Full systems considerations for multiphoton microscopy",

abstract = "A most-important-variables analysis of practical, successful multiphoton excitation fluorescence microscopy is presented. The key strength of multiphoton imaging localization of the excitation volume-helps to decouple the excitation from the emission; the emission no longer needs to be imaged. The presentation starts with maximizing the detected signal and proceeds to the laser source considerations. The main goal is to match the instrumentation to the biological problem. The main aspects covered are… Emission collection: potential signal Focal plane mismatch: chromatic aberration Light detected: actual signal Live cell imaging: preservation of biological function Deep imaging: preservation of image contrast Signal production: potential limitations.",

keywords = "Confocal, Laser scanning fluorescence microscopy, Multi-photon excitation, Objective lenses",

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year = "2001",

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AU - Girkin, J. M.

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AB - A most-important-variables analysis of practical, successful multiphoton excitation fluorescence microscopy is presented. The key strength of multiphoton imaging localization of the excitation volume-helps to decouple the excitation from the emission; the emission no longer needs to be imaged. The presentation starts with maximizing the detected signal and proceeds to the laser source considerations. The main goal is to match the instrumentation to the biological problem. The main aspects covered are… Emission collection: potential signal Focal plane mismatch: chromatic aberration Light detected: actual signal Live cell imaging: preservation of biological function Deep imaging: preservation of image contrast Signal production: potential limitations.

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KW - Laser scanning fluorescence microscopy

KW - Multi-photon excitation

KW - Objective lenses

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JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

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Full systems considerations for multiphoton microscopy

Abstract

Keywords

ASJC Scopus subject areas

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