Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics

Vijay K. Samineni, Jangyeol Yoon, Kaitlyn E. Crawford, Yu Ra Jeong, Kajanna C. McKenzie, Gunchul Shin, Zhaoqian Xie, Saranya S. Sundaram, Yuhang Li, Min Young Yang, Jeonghyun Kim, Di Wu, Yeguang Xue, Xue Feng, Yonggang Huang, Aaron D. Mickle, Anthony Banks, Jeong Sook Ha, Judith P. Golden, John A Rogers & 1 others Robert W. Gereau

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The advent of optogenetic tools has allowed unprecedented insights into the organization of neuronal networks. Although recently developed technologies have enabled implementation of optogenetics for studies of brain function in freely moving, untethered animals, wireless powering and device durability pose challenges in studies of spinal cord circuits where dynamic, multidimensional motions against hard and soft surrounding tissues can lead to device degradation. We demonstrate here a fully implantable optoelectronic device powered by near-field wireless communication technology, with a thin and flexible open architecture that provides excellent mechanical durability, robust sealing against biofluid penetration and fidelity in wireless activation, thereby allowing for long-term optical stimulation of the spinal cord without constraint on the natural behaviors of the animals. The system consists of a double-layer, rectangular-shaped magnetic coil antenna connected to a microscale inorganic light-emitting diode (-ILED) on a thin, flexible probe that can be implanted just above the dura of the mouse spinal cord for effective stimulation of light-sensitive proteins expressed in neurons in the dorsal horn. Wireless optogenetic activation of TRPV1-ChR2 afferents with spinal -ILEDs causes nocifensive behaviors and robust real-time place aversion with sustained operation in animals over periods of several weeks to months. The relatively low-cost electronics required for control of the systems, together with the biocompatibility and robust operation of these devices will allow broad application of optogenetics in future studies of spinal circuits, as well as various peripheral targets, in awake, freely moving and untethered animals, where existing approaches have limited utility.

Original languageEnglish (US)
Pages (from-to)2108-2116
Number of pages9
JournalPain
Volume158
Issue number11
DOIs
StatePublished - Nov 1 2017

Fingerprint

Optogenetics
Spinal Cord Stimulation
Equipment and Supplies
Wireless Technology
Posterior Horn Cells
Light
Animal Behavior
Spinal Cord
Communication
Technology
Costs and Cost Analysis
Brain
Proteins

Keywords

  • ArchT
  • Archaerhodopsin
  • Biocompatible
  • ChR2
  • Channelrhodopsin
  • Dorsal horn
  • Freely moving
  • Halorhodopsin
  • Itch
  • Light
  • NFC
  • NpHr3.0
  • Optoelectronic
  • Optogenetic
  • Optogenetics
  • PAIN
  • Peripheral
  • Spinal
  • Wireless

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

Samineni, V. K., Yoon, J., Crawford, K. E., Jeong, Y. R., McKenzie, K. C., Shin, G., ... Gereau, R. W. (2017). Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics. Pain, 158(11), 2108-2116. https://doi.org/10.1097/j.pain.0000000000000968
Samineni, Vijay K. ; Yoon, Jangyeol ; Crawford, Kaitlyn E. ; Jeong, Yu Ra ; McKenzie, Kajanna C. ; Shin, Gunchul ; Xie, Zhaoqian ; Sundaram, Saranya S. ; Li, Yuhang ; Yang, Min Young ; Kim, Jeonghyun ; Wu, Di ; Xue, Yeguang ; Feng, Xue ; Huang, Yonggang ; Mickle, Aaron D. ; Banks, Anthony ; Ha, Jeong Sook ; Golden, Judith P. ; Rogers, John A ; Gereau, Robert W. / Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics. In: Pain. 2017 ; Vol. 158, No. 11. pp. 2108-2116.
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Samineni, VK, Yoon, J, Crawford, KE, Jeong, YR, McKenzie, KC, Shin, G, Xie, Z, Sundaram, SS, Li, Y, Yang, MY, Kim, J, Wu, D, Xue, Y, Feng, X, Huang, Y, Mickle, AD, Banks, A, Ha, JS, Golden, JP, Rogers, JA & Gereau, RW 2017, 'Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics', Pain, vol. 158, no. 11, pp. 2108-2116. https://doi.org/10.1097/j.pain.0000000000000968

Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics. / Samineni, Vijay K.; Yoon, Jangyeol; Crawford, Kaitlyn E.; Jeong, Yu Ra; McKenzie, Kajanna C.; Shin, Gunchul; Xie, Zhaoqian; Sundaram, Saranya S.; Li, Yuhang; Yang, Min Young; Kim, Jeonghyun; Wu, Di; Xue, Yeguang; Feng, Xue; Huang, Yonggang; Mickle, Aaron D.; Banks, Anthony; Ha, Jeong Sook; Golden, Judith P.; Rogers, John A; Gereau, Robert W.

In: Pain, Vol. 158, No. 11, 01.11.2017, p. 2108-2116.

Research output: Contribution to journalArticle

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T1 - Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics

AU - Samineni, Vijay K.

AU - Yoon, Jangyeol

AU - Crawford, Kaitlyn E.

AU - Jeong, Yu Ra

AU - McKenzie, Kajanna C.

AU - Shin, Gunchul

AU - Xie, Zhaoqian

AU - Sundaram, Saranya S.

AU - Li, Yuhang

AU - Yang, Min Young

AU - Kim, Jeonghyun

AU - Wu, Di

AU - Xue, Yeguang

AU - Feng, Xue

AU - Huang, Yonggang

AU - Mickle, Aaron D.

AU - Banks, Anthony

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AU - Gereau, Robert W.

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N2 - The advent of optogenetic tools has allowed unprecedented insights into the organization of neuronal networks. Although recently developed technologies have enabled implementation of optogenetics for studies of brain function in freely moving, untethered animals, wireless powering and device durability pose challenges in studies of spinal cord circuits where dynamic, multidimensional motions against hard and soft surrounding tissues can lead to device degradation. We demonstrate here a fully implantable optoelectronic device powered by near-field wireless communication technology, with a thin and flexible open architecture that provides excellent mechanical durability, robust sealing against biofluid penetration and fidelity in wireless activation, thereby allowing for long-term optical stimulation of the spinal cord without constraint on the natural behaviors of the animals. The system consists of a double-layer, rectangular-shaped magnetic coil antenna connected to a microscale inorganic light-emitting diode (-ILED) on a thin, flexible probe that can be implanted just above the dura of the mouse spinal cord for effective stimulation of light-sensitive proteins expressed in neurons in the dorsal horn. Wireless optogenetic activation of TRPV1-ChR2 afferents with spinal -ILEDs causes nocifensive behaviors and robust real-time place aversion with sustained operation in animals over periods of several weeks to months. The relatively low-cost electronics required for control of the systems, together with the biocompatibility and robust operation of these devices will allow broad application of optogenetics in future studies of spinal circuits, as well as various peripheral targets, in awake, freely moving and untethered animals, where existing approaches have limited utility.

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KW - Itch

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KW - NFC

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Samineni VK, Yoon J, Crawford KE, Jeong YR, McKenzie KC, Shin G et al. Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics. Pain. 2017 Nov 1;158(11):2108-2116. https://doi.org/10.1097/j.pain.0000000000000968