Function, structure, and mechanism of intracellular copper trafficking proteins

D. L. Huffman*, Thomas V O'Halloran

*Corresponding author for this work

Research output: Contribution to journalReview article

384 Scopus citations

Abstract

Genetic, biochemical, and spectroscopic studies have established a new function for an intracellular protein, i.e., guiding and inserting a copper cofactor into the active site of a target enzyme. Studies of these new proteins have revealed a fundamental aspect of copper physiology, namely the vast overcapacity of the cytoplasm for copper sequestration. This finding framed the mechanistic, energetic, and structural aspects of intracellular copper trafficking proteins. One hallmark of the copper chaperones is the similarity of the protein fold between the chaperone and its target enzyme. The surface residues presented by each partner, however, are quite different, and some initial findings concerning the complementarity of these interfaces have led to mechanistic insights. The copper chaperones appear to lower the activation barrier for metal transfer into specific protein-binding sites. The manner in which they facilitate metal insertion appears to involve a docking of the metal donor and acceptor sites in close proximity to one another. Although the intimate mechanism is still open, it appears that a low activation barrier for metal transfer is achieved by a network of coordinate-covalent, electrostatic, and hydrogen bonding interactions in the vicinity of the metal-binding site itself.

Original languageEnglish (US)
Pages (from-to)677-701
Number of pages25
JournalAnnual review of biochemistry
Volume70
DOIs
StatePublished - Jul 30 2001

Keywords

  • Copper delivery
  • Menkes and Wilson disease proteins
  • Metal transfer
  • Metallochaperone
  • P-type ATPase
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Function, structure, and mechanism of intracellular copper trafficking proteins'. Together they form a unique fingerprint.

  • Cite this