TY - JOUR
T1 - Functional analysis of the multi-copper oxidase from Legionella pneumophila
AU - Huston, Wilhelmina M.
AU - Naylor, Jennifer
AU - Cianciotto, Nicholas P.
AU - Jennings, Michael P.
AU - McEwan, Alastair G.
N1 - Funding Information:
W.M.H. was supported by an Australian Postgraduate Award and thanks The University of Queensland Graduate School for a Travel Award. Part of this work was supported by a NIH grant AI34937 awarded to N.P.C. and Program Grant 284214 from the National Health and Medical Research Council of Australia to A.G.M. and M.P.J.
PY - 2008/4
Y1 - 2008/4
N2 - Multicopper oxidases have been described to have functions in copper tolerance, manganese oxidation, and iron oxidation in a range of bacteria. The putative cytoplasmic membrane multicopper oxidase from Legionella pneumophila was investigated. The mcoL gene was found to be critical for aerobic extracellular growth under either iron-limiting conditions or in the presence of ferrous Fe(II) iron, as a sole source of this essential metal. The mcoL mutants showed minor growth defects when grown in the presence of Fe(III) as the iron source. In contrast, intracellular growth and survival was not affected by the absence of the mcoL gene regardless of available iron concentration. The evidence presented here could indicate a possible role for mcoL in prevention of the toxic effects of ferrous iron during aerobic conditions. However, a function in high-affinity acquisition of iron could also be possible given the inability of the McoL mutants to grow aerobically under iron-limiting conditions.
AB - Multicopper oxidases have been described to have functions in copper tolerance, manganese oxidation, and iron oxidation in a range of bacteria. The putative cytoplasmic membrane multicopper oxidase from Legionella pneumophila was investigated. The mcoL gene was found to be critical for aerobic extracellular growth under either iron-limiting conditions or in the presence of ferrous Fe(II) iron, as a sole source of this essential metal. The mcoL mutants showed minor growth defects when grown in the presence of Fe(III) as the iron source. In contrast, intracellular growth and survival was not affected by the absence of the mcoL gene regardless of available iron concentration. The evidence presented here could indicate a possible role for mcoL in prevention of the toxic effects of ferrous iron during aerobic conditions. However, a function in high-affinity acquisition of iron could also be possible given the inability of the McoL mutants to grow aerobically under iron-limiting conditions.
KW - Iron
KW - Legionella
KW - Multicopper oxidase
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U2 - 10.1016/j.micinf.2008.01.011
DO - 10.1016/j.micinf.2008.01.011
M3 - Article
C2 - 18403241
AN - SCOPUS:43649106898
VL - 10
SP - 497
EP - 503
JO - Microbes and Infection
JF - Microbes and Infection
SN - 1286-4579
IS - 5
ER -