Functional assays of membrane-bound proteins with SAMDI-TOF mass spectrometry

Violeta L. Marin*, Timothy H. Bayburt, Stephen G. Sligar, Milan Mrksich

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


(Chemical Equation Presented) Delicate probing: When nanodisc assemblies are used to present membrane proteins on a biochip surface, interactions of these proteins can be studied by mass spectrometry. The method is illustrated with the protein rhodopsin, which is immobilized to a self-assembled monolayer by way of a his-tagged membrane scaffold protein. Upon activation of rhodopsin with light, the protein complex transducin binds and can be detected using SAMDI mass spectrometry.

Original languageEnglish (US)
Pages (from-to)8796-8798
Number of pages3
JournalAngewandte Chemie - International Edition
Issue number46
StatePublished - 2007


  • Biochips
  • Mass spectrometry
  • Monolayers
  • Proteins
  • Rhodopsin

ASJC Scopus subject areas

  • General Chemistry
  • Catalysis


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