Functional assays of membrane-bound proteins with SAMDI-TOF mass spectrometry

Violeta L. Marin; Timothy H. Bayburt; Stephen G. Sligar; Milan Mrksich

doi:10.1002/anie.200702694

Functional assays of membrane-bound proteins with SAMDI-TOF mass spectrometry

Violeta L. Marin^*, Timothy H. Bayburt, Stephen G. Sligar, Milan Mrksich

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

(Chemical Equation Presented) Delicate probing: When nanodisc assemblies are used to present membrane proteins on a biochip surface, interactions of these proteins can be studied by mass spectrometry. The method is illustrated with the protein rhodopsin, which is immobilized to a self-assembled monolayer by way of a his-tagged membrane scaffold protein. Upon activation of rhodopsin with light, the protein complex transducin binds and can be detected using SAMDI mass spectrometry.

Original language	English (US)
Pages (from-to)	8796-8798
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	46
Issue number	46
DOIs	https://doi.org/10.1002/anie.200702694
State	Published - 2007

Funding

This work was supported by the National Heart, Lung, and Blood Institute (grants HL47887, HL47889, HL47890, HL47892, HL47902, HL55208, and R01 HL58329) and the General Clinic Research Centers Program (grants NCRR GCRC, M01 RR431, and M01 RR01346).

Keywords

Biochips
Mass spectrometry
Monolayers
Proteins
Rhodopsin

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200702694

Cite this

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title = "Functional assays of membrane-bound proteins with SAMDI-TOF mass spectrometry",

abstract = "(Chemical Equation Presented) Delicate probing: When nanodisc assemblies are used to present membrane proteins on a biochip surface, interactions of these proteins can be studied by mass spectrometry. The method is illustrated with the protein rhodopsin, which is immobilized to a self-assembled monolayer by way of a his-tagged membrane scaffold protein. Upon activation of rhodopsin with light, the protein complex transducin binds and can be detected using SAMDI mass spectrometry.",

keywords = "Biochips, Mass spectrometry, Monolayers, Proteins, Rhodopsin",

author = "Marin, {Violeta L.} and Bayburt, {Timothy H.} and Sligar, {Stephen G.} and Milan Mrksich",

note = "Funding Information: This work was supported by the National Heart, Lung, and Blood Institute (grants HL47887, HL47889, HL47890, HL47892, HL47902, HL55208, and R01 HL58329) and the General Clinic Research Centers Program (grants NCRR GCRC, M01 RR431, and M01 RR01346).",

year = "2007",

doi = "10.1002/anie.200702694",

language = "English (US)",

volume = "46",

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journal = "Angewandte Chemie - International Edition",

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T1 - Functional assays of membrane-bound proteins with SAMDI-TOF mass spectrometry

AU - Marin, Violeta L.

AU - Bayburt, Timothy H.

AU - Sligar, Stephen G.

AU - Mrksich, Milan

N1 - Funding Information: This work was supported by the National Heart, Lung, and Blood Institute (grants HL47887, HL47889, HL47890, HL47892, HL47902, HL55208, and R01 HL58329) and the General Clinic Research Centers Program (grants NCRR GCRC, M01 RR431, and M01 RR01346).

PY - 2007

Y1 - 2007

N2 - (Chemical Equation Presented) Delicate probing: When nanodisc assemblies are used to present membrane proteins on a biochip surface, interactions of these proteins can be studied by mass spectrometry. The method is illustrated with the protein rhodopsin, which is immobilized to a self-assembled monolayer by way of a his-tagged membrane scaffold protein. Upon activation of rhodopsin with light, the protein complex transducin binds and can be detected using SAMDI mass spectrometry.

AB - (Chemical Equation Presented) Delicate probing: When nanodisc assemblies are used to present membrane proteins on a biochip surface, interactions of these proteins can be studied by mass spectrometry. The method is illustrated with the protein rhodopsin, which is immobilized to a self-assembled monolayer by way of a his-tagged membrane scaffold protein. Upon activation of rhodopsin with light, the protein complex transducin binds and can be detected using SAMDI mass spectrometry.

KW - Biochips

KW - Mass spectrometry

KW - Monolayers

KW - Proteins

KW - Rhodopsin

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Functional assays of membrane-bound proteins with SAMDI-TOF mass spectrometry

Abstract

Funding

Keywords

ASJC Scopus subject areas

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