Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae

Julie A. Anderson; Shirish S. Huprikar; Leon V. Kochian; William J. Lucas; Richard F. Gaber

doi:10.1073/pnas.89.9.3736

Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae

Julie A. Anderson, Shirish S. Huprikar, Leon V. Kochian, William J. Lucas, Richard F. Gaber^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

519 Scopus citations

Abstract

We report the isolation of a cDNA (KAT1) from Arabidopsis thaliana that encodes a probable K⁺ channel. KAT1 was cloned by its ability to suppress a K⁺ transport-defective phenotype in mutant Saccharomyces cerevisiae cells. This suppression is sensitive to known K⁺ channel blockers, including tetraethylammonium and Ba²⁺ ions. The KAT1 cDNA contains an open reading frame capable of encoding a 78-kDa protein that shares structural features found in the Shaker super-family of K⁺ channels. These include a cluster of six putative membrane-spanning helices (S1-S6) at the amino terminus of the protein, a presumed voltage-sensing region containing Arg/Lys-Xaa-Xaa-Arg/Lys repeats within S4, and the highly conserved pore-forming region (known as H5 or SS1-SS2). Our results suggest that the structural motif for K⁺ channels has been conserved between plants and animals.

Original language	English (US)
Pages (from-to)	3736-3740
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	9
DOIs	https://doi.org/10.1073/pnas.89.9.3736
State	Published - 1992

Keywords

Amino acid homology
Heterologous expression
Nucleotide sequence
Potassium transport
Transmembrane segments

ASJC Scopus subject areas

General

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abstract = "We report the isolation of a cDNA (KAT1) from Arabidopsis thaliana that encodes a probable K+ channel. KAT1 was cloned by its ability to suppress a K+ transport-defective phenotype in mutant Saccharomyces cerevisiae cells. This suppression is sensitive to known K+ channel blockers, including tetraethylammonium and Ba2+ ions. The KAT1 cDNA contains an open reading frame capable of encoding a 78-kDa protein that shares structural features found in the Shaker super-family of K+ channels. These include a cluster of six putative membrane-spanning helices (S1-S6) at the amino terminus of the protein, a presumed voltage-sensing region containing Arg/Lys-Xaa-Xaa-Arg/Lys repeats within S4, and the highly conserved pore-forming region (known as H5 or SS1-SS2). Our results suggest that the structural motif for K+ channels has been conserved between plants and animals.",

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Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae. / Anderson, Julie A.; Huprikar, Shirish S.; Kochian, Leon V.; Lucas, William J.; Gaber, Richard F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 9, 1992, p. 3736-3740.

Research output: Contribution to journal › Article › peer-review

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T1 - Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae

AU - Anderson, Julie A.

AU - Huprikar, Shirish S.

AU - Kochian, Leon V.

AU - Lucas, William J.

AU - Gaber, Richard F.

PY - 1992

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N2 - We report the isolation of a cDNA (KAT1) from Arabidopsis thaliana that encodes a probable K+ channel. KAT1 was cloned by its ability to suppress a K+ transport-defective phenotype in mutant Saccharomyces cerevisiae cells. This suppression is sensitive to known K+ channel blockers, including tetraethylammonium and Ba2+ ions. The KAT1 cDNA contains an open reading frame capable of encoding a 78-kDa protein that shares structural features found in the Shaker super-family of K+ channels. These include a cluster of six putative membrane-spanning helices (S1-S6) at the amino terminus of the protein, a presumed voltage-sensing region containing Arg/Lys-Xaa-Xaa-Arg/Lys repeats within S4, and the highly conserved pore-forming region (known as H5 or SS1-SS2). Our results suggest that the structural motif for K+ channels has been conserved between plants and animals.

AB - We report the isolation of a cDNA (KAT1) from Arabidopsis thaliana that encodes a probable K+ channel. KAT1 was cloned by its ability to suppress a K+ transport-defective phenotype in mutant Saccharomyces cerevisiae cells. This suppression is sensitive to known K+ channel blockers, including tetraethylammonium and Ba2+ ions. The KAT1 cDNA contains an open reading frame capable of encoding a 78-kDa protein that shares structural features found in the Shaker super-family of K+ channels. These include a cluster of six putative membrane-spanning helices (S1-S6) at the amino terminus of the protein, a presumed voltage-sensing region containing Arg/Lys-Xaa-Xaa-Arg/Lys repeats within S4, and the highly conserved pore-forming region (known as H5 or SS1-SS2). Our results suggest that the structural motif for K+ channels has been conserved between plants and animals.

KW - Amino acid homology

KW - Heterologous expression

KW - Nucleotide sequence

KW - Potassium transport

KW - Transmembrane segments

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Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae

Abstract

Keywords

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