Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae

Julie A. Anderson, Shirish S. Huprikar, Leon V. Kochian, William J. Lucas, Richard F. Gaber*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

519 Scopus citations

Abstract

We report the isolation of a cDNA (KAT1) from Arabidopsis thaliana that encodes a probable K+ channel. KAT1 was cloned by its ability to suppress a K+ transport-defective phenotype in mutant Saccharomyces cerevisiae cells. This suppression is sensitive to known K+ channel blockers, including tetraethylammonium and Ba2+ ions. The KAT1 cDNA contains an open reading frame capable of encoding a 78-kDa protein that shares structural features found in the Shaker super-family of K+ channels. These include a cluster of six putative membrane-spanning helices (S1-S6) at the amino terminus of the protein, a presumed voltage-sensing region containing Arg/Lys-Xaa-Xaa-Arg/Lys repeats within S4, and the highly conserved pore-forming region (known as H5 or SS1-SS2). Our results suggest that the structural motif for K+ channels has been conserved between plants and animals.

Original languageEnglish (US)
Pages (from-to)3736-3740
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number9
DOIs
StatePublished - 1992

Keywords

  • Amino acid homology
  • Heterologous expression
  • Nucleotide sequence
  • Potassium transport
  • Transmembrane segments

ASJC Scopus subject areas

  • General

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