Functional insights into the interplay between DNA interaction and metal coordination in ferric uptake regulators

Sabina Sarvan, François Charih, Momen Askoura, James Butcher, Joseph S Brunzelle, Alain Stintzi, Jean François Couture*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ferric uptake regulators (Fur) are a family of transcription factors coupling gene regulatory events to metal concentration. Recent evidence has expanded the mechanistic repertoires employed by Fur to activate or repress gene expression in the presence or absence of regulatory metals. However, the mechanistic basis underlying this extended repertoire has remained largely unexplored. In this study, we used an extensive set of mutations to demonstrate that Campylobacter jejuni Fur (CjFur) employs the same surface to positively and negatively control gene expression regardless of the presence or absence of metals. Moreover, the crystal structure determination of a CjFur devoid of any regulatory metals shows that subtle reorientation of the transcription factor DNA binding domain negatively impacts DNA binding, gene expression and gut colonization in chickens. Overall, these results highlight the versatility of the CjFur DNA binding domain in mediating all gene regulatory events controlled by the metalloregulator and that the full metalation of CjFur is critical to the Campylobacter jejuni life cycle in vivo.

Original languageEnglish (US)
Article number7140
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Campylobacter jejuni
Metals
DNA
Regulator Genes
Gene Expression
Transcription Factors
Life Cycle Stages
Chickens
Mutation

ASJC Scopus subject areas

  • General

Cite this

Sarvan, Sabina ; Charih, François ; Askoura, Momen ; Butcher, James ; Brunzelle, Joseph S ; Stintzi, Alain ; Couture, Jean François. / Functional insights into the interplay between DNA interaction and metal coordination in ferric uptake regulators. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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Functional insights into the interplay between DNA interaction and metal coordination in ferric uptake regulators. / Sarvan, Sabina; Charih, François; Askoura, Momen; Butcher, James; Brunzelle, Joseph S; Stintzi, Alain; Couture, Jean François.

In: Scientific reports, Vol. 8, No. 1, 7140, 01.12.2018.

Research output: Contribution to journalArticle

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