Functional magnetic resonance imaging in an animal model of pancreatic cancer

Robert J Lewandowski, Aaron C. Eifler, David Jason Bentrem, Johnathan C. Chung, Dingxin Wang, Gayle E Woloschak, Guang-Yu Yang, Robert Ryu, Riad Salem, Andrew Christian Larson, Reed A. Omary

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

AIM: To test the hypotheses that diffusion weighed (DW)- and transcatheter intraarterial perfusion (TRIP)- magnetic resonance imaging (MRI) can each be used to assess regional differences in tumor function in an animal pancreatic cancer model. METHODS: VX2 tumors were implanted in pancreata of 6 rabbits. MRI and digital subtraction angiography (DSA) were performed 3 wk following implantation. With a 2-French catheter secured in the rabbit's gastroduodenal artery, each rabbit was transferred to an adjacent 1.5T MRI scanner. DW- and TRIP-MRI were performed to determine if necrotic tumor core could be differentiated from viable tumor periphery. For each, we compared mean differences between tumor core/ periphery using a 2-tailed paired t -test (α = 0.05). Imaging was correlated with histopathology. RESULTS: Tumors were successfully grown in all rabbits, confirmed by necropsy. On DW-MRI, mean apparent diffusion coefficient (ADC) value was higher in necrotic tumor core (2.1 ± 0.3 mm2/s) than in viable tumor periphery (1.4 ± 0.5 mm2/s) (P < 0.05). On TRIP-MRI, mean perfusion values was higher in tumor periphery (110 ± 47 relative units) than in tumor core (66 ± 31 relative units) (P < 0.001). CONCLUSION: Functional MRI can be used to differentiate necrotic from viable tumor cells in an animal pancreatic cancer model using ADC (DW-MRI) and perfusion (TRIP-MRI) values.

Original languageEnglish (US)
Pages (from-to)3292-3298
Number of pages7
JournalWorld Journal of Gastroenterology
Volume16
Issue number26
DOIs
StatePublished - Jul 14 2010

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Pancreatic Neoplasms
Animal Models
Magnetic Resonance Imaging
Neoplasms
Magnetic Resonance Angiography
Rabbits
Diffusion Magnetic Resonance Imaging
Perfusion
Digital Subtraction Angiography
Pancreas
Catheters
Arteries

Keywords

  • Animal model
  • Functional magnetic resonance imaging
  • Pancreatic cancer

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Lewandowski, Robert J ; Eifler, Aaron C. ; Bentrem, David Jason ; Chung, Johnathan C. ; Wang, Dingxin ; Woloschak, Gayle E ; Yang, Guang-Yu ; Ryu, Robert ; Salem, Riad ; Larson, Andrew Christian ; Omary, Reed A. / Functional magnetic resonance imaging in an animal model of pancreatic cancer. In: World Journal of Gastroenterology. 2010 ; Vol. 16, No. 26. pp. 3292-3298.
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abstract = "AIM: To test the hypotheses that diffusion weighed (DW)- and transcatheter intraarterial perfusion (TRIP)- magnetic resonance imaging (MRI) can each be used to assess regional differences in tumor function in an animal pancreatic cancer model. METHODS: VX2 tumors were implanted in pancreata of 6 rabbits. MRI and digital subtraction angiography (DSA) were performed 3 wk following implantation. With a 2-French catheter secured in the rabbit's gastroduodenal artery, each rabbit was transferred to an adjacent 1.5T MRI scanner. DW- and TRIP-MRI were performed to determine if necrotic tumor core could be differentiated from viable tumor periphery. For each, we compared mean differences between tumor core/ periphery using a 2-tailed paired t -test (α = 0.05). Imaging was correlated with histopathology. RESULTS: Tumors were successfully grown in all rabbits, confirmed by necropsy. On DW-MRI, mean apparent diffusion coefficient (ADC) value was higher in necrotic tumor core (2.1 ± 0.3 mm2/s) than in viable tumor periphery (1.4 ± 0.5 mm2/s) (P < 0.05). On TRIP-MRI, mean perfusion values was higher in tumor periphery (110 ± 47 relative units) than in tumor core (66 ± 31 relative units) (P < 0.001). CONCLUSION: Functional MRI can be used to differentiate necrotic from viable tumor cells in an animal pancreatic cancer model using ADC (DW-MRI) and perfusion (TRIP-MRI) values.",
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Functional magnetic resonance imaging in an animal model of pancreatic cancer. / Lewandowski, Robert J; Eifler, Aaron C.; Bentrem, David Jason; Chung, Johnathan C.; Wang, Dingxin; Woloschak, Gayle E; Yang, Guang-Yu; Ryu, Robert; Salem, Riad; Larson, Andrew Christian; Omary, Reed A.

In: World Journal of Gastroenterology, Vol. 16, No. 26, 14.07.2010, p. 3292-3298.

Research output: Contribution to journalArticle

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T1 - Functional magnetic resonance imaging in an animal model of pancreatic cancer

AU - Lewandowski, Robert J

AU - Eifler, Aaron C.

AU - Bentrem, David Jason

AU - Chung, Johnathan C.

AU - Wang, Dingxin

AU - Woloschak, Gayle E

AU - Yang, Guang-Yu

AU - Ryu, Robert

AU - Salem, Riad

AU - Larson, Andrew Christian

AU - Omary, Reed A.

PY - 2010/7/14

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N2 - AIM: To test the hypotheses that diffusion weighed (DW)- and transcatheter intraarterial perfusion (TRIP)- magnetic resonance imaging (MRI) can each be used to assess regional differences in tumor function in an animal pancreatic cancer model. METHODS: VX2 tumors were implanted in pancreata of 6 rabbits. MRI and digital subtraction angiography (DSA) were performed 3 wk following implantation. With a 2-French catheter secured in the rabbit's gastroduodenal artery, each rabbit was transferred to an adjacent 1.5T MRI scanner. DW- and TRIP-MRI were performed to determine if necrotic tumor core could be differentiated from viable tumor periphery. For each, we compared mean differences between tumor core/ periphery using a 2-tailed paired t -test (α = 0.05). Imaging was correlated with histopathology. RESULTS: Tumors were successfully grown in all rabbits, confirmed by necropsy. On DW-MRI, mean apparent diffusion coefficient (ADC) value was higher in necrotic tumor core (2.1 ± 0.3 mm2/s) than in viable tumor periphery (1.4 ± 0.5 mm2/s) (P < 0.05). On TRIP-MRI, mean perfusion values was higher in tumor periphery (110 ± 47 relative units) than in tumor core (66 ± 31 relative units) (P < 0.001). CONCLUSION: Functional MRI can be used to differentiate necrotic from viable tumor cells in an animal pancreatic cancer model using ADC (DW-MRI) and perfusion (TRIP-MRI) values.

AB - AIM: To test the hypotheses that diffusion weighed (DW)- and transcatheter intraarterial perfusion (TRIP)- magnetic resonance imaging (MRI) can each be used to assess regional differences in tumor function in an animal pancreatic cancer model. METHODS: VX2 tumors were implanted in pancreata of 6 rabbits. MRI and digital subtraction angiography (DSA) were performed 3 wk following implantation. With a 2-French catheter secured in the rabbit's gastroduodenal artery, each rabbit was transferred to an adjacent 1.5T MRI scanner. DW- and TRIP-MRI were performed to determine if necrotic tumor core could be differentiated from viable tumor periphery. For each, we compared mean differences between tumor core/ periphery using a 2-tailed paired t -test (α = 0.05). Imaging was correlated with histopathology. RESULTS: Tumors were successfully grown in all rabbits, confirmed by necropsy. On DW-MRI, mean apparent diffusion coefficient (ADC) value was higher in necrotic tumor core (2.1 ± 0.3 mm2/s) than in viable tumor periphery (1.4 ± 0.5 mm2/s) (P < 0.05). On TRIP-MRI, mean perfusion values was higher in tumor periphery (110 ± 47 relative units) than in tumor core (66 ± 31 relative units) (P < 0.001). CONCLUSION: Functional MRI can be used to differentiate necrotic from viable tumor cells in an animal pancreatic cancer model using ADC (DW-MRI) and perfusion (TRIP-MRI) values.

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