Functional maturation of proteolipid protein 139-151-specific Th1 cells in the central nervous system in experimental autoimmune encephalomyelitis

Mani Mohindru, Bongsu Kang, Byung S. Kim

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Experimental autoimmune encephalomyelitis (EAE) is a widely adopted animal model system for studying human multiple sclerosis that affects the central nervous system (CNS). To understand the underlying pathogenic mechanisms of the autoimmune T cell response, localization, enumeration and characterization of autoreactive T cells are essential. We assessed encephalitogenic proteolipid protein epitope (PLP 139-151)-specific T cells in the periphery and CNS of SJL/J mice using MHC class II I-A s multimers during both pre-clinical and clinical phases of PLP-induced EAE in conjunction with T cell function. Our results strongly suggest that PLP 139-151-specific CD4 + T cells first expand primarily in the CNS-draining cervical lymph nodes and then migrate to the CNS. In the CNS, these PLP-specific CD4 + T cells accumulate, become activated and differentiate into effector cells that produce IFN-γ in response to the self-peptide.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalJournal of Neuroimmunology
Volume155
Issue number1-2
DOIs
StatePublished - Oct 2004

Keywords

  • Autoimmunity
  • Class II tetramers
  • EAE/MS
  • MHC
  • T cell receptor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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