Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging

Hao F. Zhang, Konstantin Maslov, George Stoica, Lihong V. Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1683 Scopus citations

Abstract

Although optical absorption is strongly associated with the physiological status of biological tissue, existing high-resolution optical imaging modalities, including confocal microscopy, two-photon microscopy and optical coherence tomography, do not sense optical absorption directly. Furthermore, optical scattering prevents these methods from imaging deeper than ∼1 mm below the tissue surface. Here we report functional photoacoustic microscopy (fPAM), which provides multiwavelength imaging of optical absorption and permits high spatial resolution beyond this depth limit with a ratio of maximum imaging depth to depth resolution greater than 100. Reflection mode, rather than orthogonal or transmission mode, is adopted because it is applicable to more anatomical sites than the others. fPAM is demonstrated with in vivo imaging of angiogenesis, melanoma, hemoglobin oxygen saturation (sO2) of single vessels in animals and total hemoglobin concentration in humans.

Original languageEnglish (US)
Pages (from-to)848-851
Number of pages4
JournalNature biotechnology
Volume24
Issue number7
DOIs
StatePublished - Jul 2006

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biotechnology
  • Biomedical Engineering

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