Functioning of the drosophila Wilms'-tumor-1-associated protein homolog, Fl(2)d, in sex-lethal-dependent alternative splicing

Jill K M Penn, Patricia Graham, Girish Deshpande, Gretchen Calhoun, Ahmad Sami Chaouki, Helen K. Salz, Paul Schedl*

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

fl(2)d, the Drosophila homolog of Wilms'-tumor-1-associated protein (WTAP), regulates the alternative splicing of Sex-lethal (Sxl), transformer (tra), and Ultrabithorax (Ubx). Although WTAP has been found in functional human spliceosomes, exactly how it contributes to the splicing process remains unknown. Here we attempt to identify factors that interact genetically and physically with fl(2)d. We begin by analyzing the Sxl-Fl(2)d protein-protein interaction in detail and present evidence suggesting that the female-specific fl(2)d1 allele is antimorphic with respect to the process of sex determination. Next we show that fl(2)d interacts genetically with early acting general splicing regulators and that Fl(2)d is present in immunoprecipitable complexes with Snf, U2AF50, U2AF38, and U1-70K. By contrast, we could not detect Fl(2)d complexes containing the U5 snRNP protein U5-40K or with a protein that associates with the activated B spliceosomal complex SKIP. Significantly, the genetic and molecular interactions observed for Sxl are quite similar to those detected for fl(2)d. Taken together, our findings suggest that Sxl and fl(2)d function to alter splice-site selection at an early step in spliceosome assembly.

Original languageEnglish (US)
Pages (from-to)737-748
Number of pages12
JournalGenetics
Volume178
Issue number2
DOIs
Publication statusPublished - Feb 1 2008

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this