Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus

R. B. Belshe, L. S. Richardson, T. J. Schnitzer, D. A. Prevar, E. Camargo, R. M. Chanock

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Abstract

ts-2, a temperature-sensitive and plaque morphology mutant of respiratory syncytial virus and sole representative of complementation group B, was compared with members of the other complementation groups of respiratory syncytial virus (group A [ts-1] and group C [ts-7]). ts-2 was found to be 10- to 1,000-fold more restricted in growth and ability to spread at restrictive temperatures (37, 38, and 39 °C) than at the permissive temperature (32°C). In temperature shift-up experiments, the ts defect of ts-1 and other members of complementation group A was found to affect a late function that was required for at least 13 h in the replication cycle. The ts lesion of ts-7 affected a function early in the replication cycle. In contrast, ts-2 was not temperature sensitive when studied by the shift-up technique. The discrepancy between the ts plaque property and failure to detect temperature sensitivity during the shift-up experiment was resolved when it was shown that ts-2 had a defect in adsorption or penetration or both at the restrictive temperature. Clonal analysis of revertant ts-2 showed a coordinate restoration of ts + phenotype and syncytium-forming capacity. It appears that ts-2 has a defect in a protein that is involved in adsorption and/or penetration of virus and is also responsible for cell fusion activity.

Original languageEnglish (US)
Pages (from-to)8-12
Number of pages5
JournalJournal of Virology
Volume24
Issue number1
Publication statusPublished - Dec 1 1977

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Belshe, R. B., Richardson, L. S., Schnitzer, T. J., Prevar, D. A., Camargo, E., & Chanock, R. M. (1977). Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus. Journal of Virology, 24(1), 8-12.