G protein coupled receptor transcripts in human immune cells and platelets

Arne Hansen; Daniel Martin; Florian Langer; Kathleen Harrison; John Kehrl; Claudia Cicala; Elena Martinelli; Michael J. Brownstein; Eva Mezey

doi:10.1038/s41597-024-03880-2

G protein coupled receptor transcripts in human immune cells and platelets

Arne Hansen, Daniel Martin^*, Florian Langer, Kathleen Harrison, John Kehrl, Claudia Cicala, Elena Martinelli, Michael J. Brownstein, Eva Mezey^*

^*Corresponding author for this work

Medicine, Infectious Diseases Division

Research output: Contribution to journal › Article › peer-review

Abstract

G-protein coupled receptors (GPCRs) are encoded by nonabundant mRNAs, and it is difficult to detect them reliably with the highly parallel methods that are in general use. Because of this, we developed and validated a sensitive, specific, semi-quantitative method for detecting these transcripts. We have used the method to profile GPCR transcripts in white blood cells (WBCs)–B, CD4, CD8, NK, and dendritic cells; monocytes, and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor–as well as platelets. On average, the white cells studied expressed 160 receptor mRNAs (range, 123–206). Platelets made 69. Some, but far from all, of the receptors we found have been detected earlier. We believe our data should stimulate studies of receptor function and contribute to drug development.

Original language	English (US)
Article number	1040
Journal	Scientific Data
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41597-024-03880-2
State	Published - Dec 2024

Funding

The project was supported by the NIH/NIDCR DIR intramural project # ZIA DE000714 and # ZIC DC000086. The authors want to thank Lynn-Vitale Cross for her help with preparing Tables and lists.

ASJC Scopus subject areas

Statistics and Probability
Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

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10.1038/s41597-024-03880-2

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abstract = "G-protein coupled receptors (GPCRs) are encoded by nonabundant mRNAs, and it is difficult to detect them reliably with the highly parallel methods that are in general use. Because of this, we developed and validated a sensitive, specific, semi-quantitative method for detecting these transcripts. We have used the method to profile GPCR transcripts in white blood cells (WBCs)–B, CD4, CD8, NK, and dendritic cells; monocytes, and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor–as well as platelets. On average, the white cells studied expressed 160 receptor mRNAs (range, 123–206). Platelets made 69. Some, but far from all, of the receptors we found have been detected earlier. We believe our data should stimulate studies of receptor function and contribute to drug development.",

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AU - Hansen, Arne

AU - Martin, Daniel

AU - Langer, Florian

AU - Harrison, Kathleen

AU - Kehrl, John

AU - Cicala, Claudia

AU - Martinelli, Elena

AU - Brownstein, Michael J.

AU - Mezey, Eva

PY - 2024/12

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G protein coupled receptor transcripts in human immune cells and platelets

Abstract

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ASJC Scopus subject areas

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