G-proteins are involved in riluzole inhibition of high voltage-activated calcium channels in rat dorsal root ganglion neurons

Chao Sheng Huang, Jin Ho Song, Keiichi Nagata, Dennis Twombly, Jay Z. Yeh, Toshio Narahashi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Effects of riluzole on high voltage-activated (HVA) calcium channels of rat dorsal root ganglion neurons were studied using the whole cell patch- clamp technique. Riluzole at 30 μM inhibited the HVA currents. The onset and offset of riluzole inhibitory effect were slow usually taking more than 3 min. Riluzole inhibition of the HVA currents was abolished and partially reduced by addition of 500 μM GDP-β-S and 1 mM N-ethylmaleimide, respectively, to the pipette solution. Pre-treatment with pertussis toxin or application of depolarizing pre-pulses did not affect riluzole's inhibitory effect on the HVA currents. Riluzole inhibition of the HVA currents was also blocked by internal application of 50 μg/ml protein kinase A inhibitory peptide. It was concluded that pertussis toxin-insensitive G-proteins and protein kinase A may be involved in riluzole inhibition of the HVA currents.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalBrain research
Volume762
Issue number1-2
DOIs
StatePublished - Jul 11 1997

Keywords

  • G-protein
  • GDP-β-S
  • High voltage- activated calcium channel
  • N-ethylmaleimide
  • Neuroprotective drug
  • Pertussis toxin
  • Protein kinase A
  • Riluzole

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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