Ganglioside GT1b induces keratinocyte differentiation without activating protein kinase C

Amy S. Paller*, Sheryl L. Arnsmeier, Gary J. Fisher, Qian Chun Yu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


The altered patterns of expression of gangliosides during density-dependent growth inhibition, oncogenic transformation, and embryogenesis suggest that gangliosides, sialylated membrane glycolipids, may affect cellular proliferation and differentiation. Gangliosides of the “b” pathway of ganglioside synthesis, including GM3, GDa, and GD1b, inhibit the proliferation of cultured keratinocytes without increasing differentiation. We have examined the effect on keratinocyte proliferation and differentiation of supplemental ganglioside CT1b, a more highly sialylated ganglioside of the “b” synthetic pathway that is also present in cultured keratinocytes. In contrast to the tack of effect on differentiation of these other gangliosides, we noted significant induction of keratinocyte differentiation by GT1b, as evidenced by early desmosome formation, and increased cornified envelope formation and expression of involucrin and of the differentiation-specific keratin K1. The addition of GT1b did not cause a shift in intracellular free calcium or alter protein kinase C activity. Alterations in the membrane concentration of ganglioside GT1b, a minor ganglioside component of the keratinocyte membrane, may participate in regulating keratinocyte differentiation.

Original languageEnglish (US)
Pages (from-to)118-124
Number of pages7
JournalExperimental Cell Research
Issue number1
StatePublished - Mar 1995

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Ganglioside GT1b induces keratinocyte differentiation without activating protein kinase C'. Together they form a unique fingerprint.

Cite this