Ganglioside G(T1b) inhibits keratinocyte adhesion and migration on a fibronectin matrix

A. S. Paller*, S. L. Arnsmeier, J. D. Chen, D. T. Woodley

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Highly sialylated gangliosides have been shown to alter cellular adhesion to a fibronectin matrix. The effect of these gangliosides on the adhesion, spreading, and migration of cultured keratinocytes on a fibronectin matrix has not been explored. Ganglioside G(T1b) significantly prevented attachment of keratinocytes to fibronectin and also detached previously adherent keratinocytes in a concentration-dependent manner without cell toxicity. G(T1b) did not affect adhesion of keratinocytes to wells coated with laminin, type I or type IV collagen, 804G extracellular matrix, or albumin. G(T1b) also inhibited keratinocyte migration on fibronectin in a concentration-dependent manner at concentrations as low as 5 nM G(T1b), but had no effect on migration of keratinocytes plated on other matrices. G(T1b) binds to intact fibronectin and to the 120-kD RGDS-containing cell binding fibronectin fragment, but not to the heparin or gelatin-binding fragments of fibronectin. Although RGDS competes with G(T1b) in inhibiting adhesion, G(T1b) does not diminish binding of keratinocytes to a derivatized RGDS substratum, suggesting that the G(T1b) effect involves a non-RGDS site in the cell-binding region that modulates RGDS/α5β1 integrin receptor interaction. Through a specific effect on keratinocyte interaction with fibronectin, G(T1b) may participate in the regulation of cell adhesion and migration on a fibronectin substratum, which are important events during wound healing and the spreading of cutaneous neoplasia.

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalJournal of Investigative Dermatology
Volume105
Issue number2
DOIs
StatePublished - 1995

Keywords

  • Glycosphingolipids
  • Integrins
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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