Gas challenge-blood oxygen level-dependent (GC-BOLD) MRI in the rat Novikoff hepatoma model

Yang Guo, Ning Jin, Rachel Klein, Jodi Nicolai, Guang Yu Yang, Reed A. Omary, Andrew C. Larson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Purpose: The purpose of the study was to investigate the relationship between gas challenge-blood oxygen level-dependent (GC-BOLD) response angiogenesis and tumor size in rat Novikoff hepatoma model. Materials and Methods: Twenty adult male Sprague-Dawley rats (weighting 301-325 g) were used for our Animal Care and Use Committee-approved experiments. N1-S1 Novikoff hepatomas were grown in 14 rats with sizes ranging from 0.42 to 2.81 cm. All experiments were performed at 3.0 T using a custom-built rodent receiver coil. A multiple gradient-echo sequence was used for R2 n measurements, first during room air (78% N 2/20% O 2) breathing and then after 10 min of carbogen (95% O 2/5% CO 2) breathing. After image acquisition, rats were euthanized, and the tumors were harvested for histological evaluation. Results: The R2 n change between air and carbogen breathing for small hepatomas was positive; R2 n changes changed to negative values for larger hepatomas. We found a significant positive correlation between tumor R2 n change and tumor microvessel density (MVD) (r=0.798, P=001) and a significant inverse correlation between tumor R2 n change and tumor size (r=-0.840, P<.0001). Conclusions: GC-BOLD magnetic resonance imaging measurements are well correlated to MVD levels and tumor size in the N1-S1 Novikoff hepatoma model; GC-BOLD measurements may serve as noninvasive biomarkers for evaluating angiogenesis and disease progression and/or therapy response.

Original languageEnglish (US)
Pages (from-to)133-138
Number of pages6
JournalMagnetic Resonance Imaging
Volume30
Issue number1
DOIs
StatePublished - Jan 2012

Funding

This publication was made possible by grant number CA134719 from the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NIH.

Keywords

  • Gas challenge-blood oxygen level dependent (GC-BOLD)
  • MRI
  • Rat Novikoff hepatoma
  • Tumor angiogenesis
  • Tumor microvessel density

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Biomedical Engineering

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