GAS6 is an estrogen-inducible gene in mammary epithelial cells

Rigen Mo; Yiwei Tony Zhu; Zhongyi Zhang; Sambasiva M. Rao; Yi Jun Zhu

doi:10.1016/j.bbrc.2006.12.018

GAS6 is an estrogen-inducible gene in mammary epithelial cells

Rigen Mo, Yiwei Tony Zhu, Zhongyi Zhang, Sambasiva M. Rao, Yi Jun Zhu^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

To identify estrogen-responsive genes in mammary glands, microarray assays were performed. Twenty genes were found to be up-regulated while 16 genes were repressed in the 9 h estrogen treated glands. The induction of GAS6, one of the genes up-regulated by estrogen, was confirmed by RNase protection assay. Furthermore, GAS6 was also demonstrated to be induced by estrogen in ER positive breast cancer cells. Analysis of GAS6 promoter revealed that GAS6 promoter was regulated by estrogen. An estrogen response element (ERE) was identified in the GAS6 promoter. Electrophoretic mobility shift assay revealed that ERα interacted with the ERE in the GAS6 promoter. Chromatin immunoprecipitation demonstrated that ERα was recruited to the GAS6 promoter upon estrogen stimulation. These results suggested that GAS6 is an estrogen target gene in mammary epithelial cells.

Original language	English (US)
Pages (from-to)	189-194
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	353
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.12.018
State	Published - Feb 2 2007

Keywords

Estrogen
Estrogen receptor
GAS6
Mammary gland

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.bbrc.2006.12.018

Cite this

@article{f357c0d1d99b4f1981c13a01f446fda2,

title = "GAS6 is an estrogen-inducible gene in mammary epithelial cells",

abstract = "To identify estrogen-responsive genes in mammary glands, microarray assays were performed. Twenty genes were found to be up-regulated while 16 genes were repressed in the 9 h estrogen treated glands. The induction of GAS6, one of the genes up-regulated by estrogen, was confirmed by RNase protection assay. Furthermore, GAS6 was also demonstrated to be induced by estrogen in ER positive breast cancer cells. Analysis of GAS6 promoter revealed that GAS6 promoter was regulated by estrogen. An estrogen response element (ERE) was identified in the GAS6 promoter. Electrophoretic mobility shift assay revealed that ERα interacted with the ERE in the GAS6 promoter. Chromatin immunoprecipitation demonstrated that ERα was recruited to the GAS6 promoter upon estrogen stimulation. These results suggested that GAS6 is an estrogen target gene in mammary epithelial cells.",

keywords = "Estrogen, Estrogen receptor, GAS6, Mammary gland",

author = "Rigen Mo and {Tony Zhu}, Yiwei and Zhongyi Zhang and Rao, {Sambasiva M.} and Zhu, {Yi Jun}",

note = "Funding Information: This work was supported by National Institutes of Health Grant CA 88898 (To Y.J.Z.). ",

year = "2007",

month = feb,

day = "2",

doi = "10.1016/j.bbrc.2006.12.018",

language = "English (US)",

volume = "353",

pages = "189--194",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - GAS6 is an estrogen-inducible gene in mammary epithelial cells

AU - Mo, Rigen

AU - Tony Zhu, Yiwei

AU - Zhang, Zhongyi

AU - Rao, Sambasiva M.

AU - Zhu, Yi Jun

N1 - Funding Information: This work was supported by National Institutes of Health Grant CA 88898 (To Y.J.Z.).

PY - 2007/2/2

Y1 - 2007/2/2

N2 - To identify estrogen-responsive genes in mammary glands, microarray assays were performed. Twenty genes were found to be up-regulated while 16 genes were repressed in the 9 h estrogen treated glands. The induction of GAS6, one of the genes up-regulated by estrogen, was confirmed by RNase protection assay. Furthermore, GAS6 was also demonstrated to be induced by estrogen in ER positive breast cancer cells. Analysis of GAS6 promoter revealed that GAS6 promoter was regulated by estrogen. An estrogen response element (ERE) was identified in the GAS6 promoter. Electrophoretic mobility shift assay revealed that ERα interacted with the ERE in the GAS6 promoter. Chromatin immunoprecipitation demonstrated that ERα was recruited to the GAS6 promoter upon estrogen stimulation. These results suggested that GAS6 is an estrogen target gene in mammary epithelial cells.

AB - To identify estrogen-responsive genes in mammary glands, microarray assays were performed. Twenty genes were found to be up-regulated while 16 genes were repressed in the 9 h estrogen treated glands. The induction of GAS6, one of the genes up-regulated by estrogen, was confirmed by RNase protection assay. Furthermore, GAS6 was also demonstrated to be induced by estrogen in ER positive breast cancer cells. Analysis of GAS6 promoter revealed that GAS6 promoter was regulated by estrogen. An estrogen response element (ERE) was identified in the GAS6 promoter. Electrophoretic mobility shift assay revealed that ERα interacted with the ERE in the GAS6 promoter. Chromatin immunoprecipitation demonstrated that ERα was recruited to the GAS6 promoter upon estrogen stimulation. These results suggested that GAS6 is an estrogen target gene in mammary epithelial cells.

KW - Estrogen

KW - Estrogen receptor

KW - GAS6

KW - Mammary gland

UR - http://www.scopus.com/inward/record.url?scp=33845634396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845634396&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.12.018

DO - 10.1016/j.bbrc.2006.12.018

M3 - Article

C2 - 17174935

AN - SCOPUS:33845634396

SN - 0006-291X

VL - 353

SP - 189

EP - 194

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

GAS6 is an estrogen-inducible gene in mammary epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this