GAS6 is an estrogen-inducible gene in mammary epithelial cells

Rigen Mo, Yiwei Tony Zhu, Zhongyi Zhang, Sambasiva M. Rao, Yi Jun Zhu*

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

To identify estrogen-responsive genes in mammary glands, microarray assays were performed. Twenty genes were found to be up-regulated while 16 genes were repressed in the 9 h estrogen treated glands. The induction of GAS6, one of the genes up-regulated by estrogen, was confirmed by RNase protection assay. Furthermore, GAS6 was also demonstrated to be induced by estrogen in ER positive breast cancer cells. Analysis of GAS6 promoter revealed that GAS6 promoter was regulated by estrogen. An estrogen response element (ERE) was identified in the GAS6 promoter. Electrophoretic mobility shift assay revealed that ERα interacted with the ERE in the GAS6 promoter. Chromatin immunoprecipitation demonstrated that ERα was recruited to the GAS6 promoter upon estrogen stimulation. These results suggested that GAS6 is an estrogen target gene in mammary epithelial cells.

Original languageEnglish (US)
Pages (from-to)189-194
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume353
Issue number1
DOIs
Publication statusPublished - Feb 2 2007

    Fingerprint

Keywords

  • Estrogen
  • Estrogen receptor
  • GAS6
  • Mammary gland

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this