Gaussian Beam optical parameters in multi-photon excitation fluorescence imaging

Lingyan Shi, Adrián Rodríguez-Contreras, Robert R. Alfano

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


This chapter inspects and compares the critical optical properties of Gaussian laser beam in theory, including the beam spot size, depth of focus, and intensity profile, in two-photon and multiphoton fluorescence imaging for quantifying nanometer-scale solutes.

Original languageEnglish (US)
Title of host publicationDeep Imaging in Tissue and Biomedical Materials
Subtitle of host publicationUsing Linear and Nonlinear Optical Methods
PublisherPan Stanford Publishing Pte. Ltd.
Number of pages10
ISBN (Electronic)9781351797399
ISBN (Print)9789814745888
StatePublished - Jan 1 2017

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)


Dive into the research topics of 'Gaussian Beam optical parameters in multi-photon excitation fluorescence imaging'. Together they form a unique fingerprint.

Cite this