TY - JOUR
T1 - Gd-EOB-DTPA DCE-MRI biomarkers in a rabbit model of liver fibrosis
AU - Ji, Yang
AU - Zhang, Chuanshan
AU - Huang, Zhe
AU - Wang, Xia
AU - Yue, Lina
AU - Gao, Meimei
AU - Hu, Huiling
AU - Su, Qinjun
AU - Han, Yuedong
AU - Liu, Bin
AU - Yang, Ding
AU - Su, Zhanliang
AU - Zhang, Zhuoli
N1 - Funding Information:
This work was supported by natural science foundation research project by Shaanxi province (No: S2015YFJM1473) and grants R01CA-196967 and R01CA209886 funded by the USA National Cancer Institute (National Institutes of Health).
Publisher Copyright:
© 2018, E-Century Publishing Corporation. All rights reserved.
PY - 2018
Y1 - 2018
N2 - The purpose of this study was to investigate the correlations between Gd-EOB-DTPA DCE-MRI biomarkers and histopathologic biomarkers of liver fibrosis progression in a rabbit model of liver fibrosis. Thirty-Six New Zealand white rabbits were randomly divided into control (n = 6) and liver fibrosis group (n = 30). Each rabbit in the liver fibrosis group received a weekly subcutaneous injection in the back comprising 50% carbon tetrachloride (CCl4) in oily solution. Control rabbits received subcutaneous injections with the same amount of normal saline solution instead. MR imaging was performed in control and fibrotic rabbits were conducted by MRI at week 0 (n = 36). The fibrotic rabbits were performed MR imaging on 6 weeks, 9 weeks, and 12 weeks after modeling of fibrosis. Before each MRI, peripheral blood was collected, and several biochemical testes are performed. The thirty-four rabbits completed this study. They were then divided into three subgroups according to fibrotic stage: no fibrosis (F0, n = 12), mild fibrosis (F1+F2, n = 14), and advanced fibrosis (F3+F4, n = 8). DCE-MRI measurements show increasing Ktrans and Ve while decreasing iACU90 with increasing fibrosis stage. The significant correlations were observed between mean Ktrans, Ve, iACU90 and percentage of the animal with mild liver fibrosis. For blood biomarkers, there were significant differences between F0 and F1+F2, and between F0 and F3+F4 in the serum levels of ALT (all P < 0.05), and TB (F0 vs. F1+F2, P = 0.004), while no differences were found between F1+F2 group and F3+F4 group (all P > 0.05). There were significant differences between F0 and F1+F2 (P = 0.02). Gd-EOB-DTPA DCE-MRI is a promising method for the noninvasive diagnosis and staging of liver fibrosis. Future studies to evaluate the effectiveness of these techniques in patients with liver fibrosis are warranted.
AB - The purpose of this study was to investigate the correlations between Gd-EOB-DTPA DCE-MRI biomarkers and histopathologic biomarkers of liver fibrosis progression in a rabbit model of liver fibrosis. Thirty-Six New Zealand white rabbits were randomly divided into control (n = 6) and liver fibrosis group (n = 30). Each rabbit in the liver fibrosis group received a weekly subcutaneous injection in the back comprising 50% carbon tetrachloride (CCl4) in oily solution. Control rabbits received subcutaneous injections with the same amount of normal saline solution instead. MR imaging was performed in control and fibrotic rabbits were conducted by MRI at week 0 (n = 36). The fibrotic rabbits were performed MR imaging on 6 weeks, 9 weeks, and 12 weeks after modeling of fibrosis. Before each MRI, peripheral blood was collected, and several biochemical testes are performed. The thirty-four rabbits completed this study. They were then divided into three subgroups according to fibrotic stage: no fibrosis (F0, n = 12), mild fibrosis (F1+F2, n = 14), and advanced fibrosis (F3+F4, n = 8). DCE-MRI measurements show increasing Ktrans and Ve while decreasing iACU90 with increasing fibrosis stage. The significant correlations were observed between mean Ktrans, Ve, iACU90 and percentage of the animal with mild liver fibrosis. For blood biomarkers, there were significant differences between F0 and F1+F2, and between F0 and F3+F4 in the serum levels of ALT (all P < 0.05), and TB (F0 vs. F1+F2, P = 0.004), while no differences were found between F1+F2 group and F3+F4 group (all P > 0.05). There were significant differences between F0 and F1+F2 (P = 0.02). Gd-EOB-DTPA DCE-MRI is a promising method for the noninvasive diagnosis and staging of liver fibrosis. Future studies to evaluate the effectiveness of these techniques in patients with liver fibrosis are warranted.
KW - Animal
KW - Liver fibrosis
KW - Magnetic resonance imaging
KW - Pathology
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M3 - Article
AN - SCOPUS:85054552941
SN - 1943-8141
VL - 10
SP - 2949
EP - 2957
JO - American Journal of Translational Research
JF - American Journal of Translational Research
IS - 9
M1 - AJTR0077047
ER -
