Gene Delivery Efficiency in Bone Marrow-derived Dendritic Cells: Comparison of Four Methods and Optimization for Lentivirus Transduction

Gong Bo Li, Guang Xiu Lu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Many gene delivery methods have been used to transduce or transfect bone marrow-derived dendritic cells (BMDCs) for genetic engineered DC vaccine research. The present study, for the first time, evaluated the efficiencies of four methods (lipofection, DNA electroporation, recombinant adeno-associated virus type 2 (rAAV2) transduction, and recombinant lentivirus (rLV) transduction) using EGFP as a report gene in the same BMDC culture system. Our data demonstrate that rLV transduction is the most effective method; both lipofection and DNA electroporation transfect BMDCs at lower efficiencies; rAAV2 can hardly transduce BMDCs. Furthermore, our results, for the first time, demonstrate that rLV transduction efficiency on BMDCs can be improved significantly by co-centrifugation and repeated transduction.

Original languageEnglish (US)
Pages (from-to)250-256
Number of pages7
JournalMolecular Biotechnology
Volume43
Issue number3
DOIs
StatePublished - Nov 2009
Externally publishedYes

Keywords

  • Dendritic cell
  • Gene delivery
  • Lentivirus

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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