Gene encoding human growth hormone-releasing factor precursor: Structure, sequence, and chromosomal assignment

K. E. Mayo, G. M. Cerelli, R. V. Lebo, B. D. Bruce, M. G. Rosenfeld, R. M. Evans

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

We have isolated and characterized overlapping clones from phage λ and cosmid human genomic libraries that predict the entire structure of the gene encoding the precursor to human growth hormone-releasing factor. The gene includes five exons spanning 10 kilobase pairs of human genomic DNA. There appears to be a segregation of distinct functional regions of the GRF precursor and its mRNA into the five exons of the gene. The DNA sequences of all exons, intron/exon boundaries, and 5' and 3' flanking regions are presented. Dot-blot analysis of DNA from high resolution dual-laser-sorted human chromosomes indicates that the single-copy growth hormone-releasing factor gene is located on human chromosome 20.

Original languageEnglish (US)
Pages (from-to)63-67
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number1
DOIs
StatePublished - Apr 10 1985

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Gene encoding human growth hormone-releasing factor precursor: Structure, sequence, and chromosomal assignment'. Together they form a unique fingerprint.

Cite this