Gene encoding human growth hormone-releasing factor precursor: Structure, sequence, and chromosomal assignment

K. E. Mayo; G. M. Cerelli; R. V. Lebo; B. D. Bruce; M. G. Rosenfeld; R. M. Evans

doi:10.1073/pnas.82.1.63

Gene encoding human growth hormone-releasing factor precursor: Structure, sequence, and chromosomal assignment

K. E. Mayo, G. M. Cerelli, R. V. Lebo, B. D. Bruce, M. G. Rosenfeld, R. M. Evans

Molecular Biosciences

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Abstract

We have isolated and characterized overlapping clones from phage λ and cosmid human genomic libraries that predict the entire structure of the gene encoding the precursor to human growth hormone-releasing factor. The gene includes five exons spanning 10 kilobase pairs of human genomic DNA. There appears to be a segregation of distinct functional regions of the GRF precursor and its mRNA into the five exons of the gene. The DNA sequences of all exons, intron/exon boundaries, and 5' and 3' flanking regions are presented. Dot-blot analysis of DNA from high resolution dual-laser-sorted human chromosomes indicates that the single-copy growth hormone-releasing factor gene is located on human chromosome 20.

Original language	English (US)
Pages (from-to)	63-67
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	82
Issue number	1
DOIs	https://doi.org/10.1073/pnas.82.1.63
State	Published - 1985

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abstract = "We have isolated and characterized overlapping clones from phage λ and cosmid human genomic libraries that predict the entire structure of the gene encoding the precursor to human growth hormone-releasing factor. The gene includes five exons spanning 10 kilobase pairs of human genomic DNA. There appears to be a segregation of distinct functional regions of the GRF precursor and its mRNA into the five exons of the gene. The DNA sequences of all exons, intron/exon boundaries, and 5' and 3' flanking regions are presented. Dot-blot analysis of DNA from high resolution dual-laser-sorted human chromosomes indicates that the single-copy growth hormone-releasing factor gene is located on human chromosome 20.",

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AU - Mayo, K. E.

AU - Cerelli, G. M.

AU - Lebo, R. V.

AU - Bruce, B. D.

AU - Rosenfeld, M. G.

AU - Evans, R. M.

PY - 1985

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AB - We have isolated and characterized overlapping clones from phage λ and cosmid human genomic libraries that predict the entire structure of the gene encoding the precursor to human growth hormone-releasing factor. The gene includes five exons spanning 10 kilobase pairs of human genomic DNA. There appears to be a segregation of distinct functional regions of the GRF precursor and its mRNA into the five exons of the gene. The DNA sequences of all exons, intron/exon boundaries, and 5' and 3' flanking regions are presented. Dot-blot analysis of DNA from high resolution dual-laser-sorted human chromosomes indicates that the single-copy growth hormone-releasing factor gene is located on human chromosome 20.

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Gene encoding human growth hormone-releasing factor precursor: Structure, sequence, and chromosomal assignment

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