Gene expression profiles in mouse liver after long-term low-dose-rate irradiation with gamma rays

Yoshihiko Uehara*, Yasuko Ito, Keiko Taki, Mitsuru Nenoi, Kazuaki Ichinohe, Shingo Nakamura, Satoshi Tanaka, Yoichi Oghiso, Kimio Tanaka, Tsuneya Matsumoto, Tatjana Paunesku, Gayle E. Woloschak, Tetsuya Ono

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Changes in gene expression profiles in mouse liver induced by long-term low-dose-rate irradiation were examined by microarray analysis. Three groups of male C57BL/6J mice were exposed to whole-body radiation at dose rates of 1720 mGy/day, 0.861.0 mGy/day or 0.0420.050 mGy/day for 401485 days with cumulative doses of approximately 8 Gy, 0.4 Gy or 0.02 Gy, respectively. The gene expression levels in the livers of six animals from each exposure group were compared individually with that of pooled sham-irradiated animals. Some genes revealed a large variation in expression levels among individuals within each group, and the number of genes showing common changes in individuals from each group was limited: 20 and 11 genes showed more than 1.5-fold modulation with 1720 mGy/day and 0.861.0 mGy/day, respectively. Three genes showed more than 1.5-fold modulation even at the lowest dose-rate of 0.040.05 mGy/day. Most of these genes were down-regulated. RT-PCR analysis confirmed the expression profiles of the majority of these genes. The results indicate that a few genes are modulated in response to very low-dose-rate irradiation. The functional analysis suggests that these genes may influence many processes, including obesity and tumorigenesis.

Original languageEnglish (US)
Pages (from-to)611-617
Number of pages7
JournalRadiation Research
Volume174
Issue number5
DOIs
StatePublished - Nov 2010

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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