Gene expression profiles in mouse liver after long-term low-dose-rate irradiation with gamma rays

Yoshihiko Uehara; Yasuko Ito; Keiko Taki; Mitsuru Nenoi; Kazuaki Ichinohe; Shingo Nakamura; Satoshi Tanaka; Yoichi Oghiso; Kimio Tanaka; Tsuneya Matsumoto; Tatjana Paunesku; Gayle E. Woloschak; Tetsuya Ono

doi:10.1667/RR2195.1

Gene expression profiles in mouse liver after long-term low-dose-rate irradiation with gamma rays

Yoshihiko Uehara^*, Yasuko Ito, Keiko Taki, Mitsuru Nenoi, Kazuaki Ichinohe, Shingo Nakamura, Satoshi Tanaka, Yoichi Oghiso, Kimio Tanaka, Tsuneya Matsumoto, Tatjana Paunesku, Gayle E. Woloschak, Tetsuya Ono

^*Corresponding author for this work

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Changes in gene expression profiles in mouse liver induced by long-term low-dose-rate irradiation were examined by microarray analysis. Three groups of male C57BL/6J mice were exposed to whole-body radiation at dose rates of 1720 mGy/day, 0.861.0 mGy/day or 0.0420.050 mGy/day for 401485 days with cumulative doses of approximately 8 Gy, 0.4 Gy or 0.02 Gy, respectively. The gene expression levels in the livers of six animals from each exposure group were compared individually with that of pooled sham-irradiated animals. Some genes revealed a large variation in expression levels among individuals within each group, and the number of genes showing common changes in individuals from each group was limited: 20 and 11 genes showed more than 1.5-fold modulation with 1720 mGy/day and 0.861.0 mGy/day, respectively. Three genes showed more than 1.5-fold modulation even at the lowest dose-rate of 0.040.05 mGy/day. Most of these genes were down-regulated. RT-PCR analysis confirmed the expression profiles of the majority of these genes. The results indicate that a few genes are modulated in response to very low-dose-rate irradiation. The functional analysis suggests that these genes may influence many processes, including obesity and tumorigenesis.

Original language	English (US)
Pages (from-to)	611-617
Number of pages	7
Journal	Radiation Research
Volume	174
Issue number	5
DOIs	https://doi.org/10.1667/RR2195.1
State	Published - Nov 2010

ASJC Scopus subject areas

Radiation
Biophysics
Radiology Nuclear Medicine and imaging

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10.1667/RR2195.1

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title = "Gene expression profiles in mouse liver after long-term low-dose-rate irradiation with gamma rays",

abstract = "Changes in gene expression profiles in mouse liver induced by long-term low-dose-rate irradiation were examined by microarray analysis. Three groups of male C57BL/6J mice were exposed to whole-body radiation at dose rates of 1720 mGy/day, 0.861.0 mGy/day or 0.0420.050 mGy/day for 401485 days with cumulative doses of approximately 8 Gy, 0.4 Gy or 0.02 Gy, respectively. The gene expression levels in the livers of six animals from each exposure group were compared individually with that of pooled sham-irradiated animals. Some genes revealed a large variation in expression levels among individuals within each group, and the number of genes showing common changes in individuals from each group was limited: 20 and 11 genes showed more than 1.5-fold modulation with 1720 mGy/day and 0.861.0 mGy/day, respectively. Three genes showed more than 1.5-fold modulation even at the lowest dose-rate of 0.040.05 mGy/day. Most of these genes were down-regulated. RT-PCR analysis confirmed the expression profiles of the majority of these genes. The results indicate that a few genes are modulated in response to very low-dose-rate irradiation. The functional analysis suggests that these genes may influence many processes, including obesity and tumorigenesis.",

author = "Yoshihiko Uehara and Yasuko Ito and Keiko Taki and Mitsuru Nenoi and Kazuaki Ichinohe and Shingo Nakamura and Satoshi Tanaka and Yoichi Oghiso and Kimio Tanaka and Tsuneya Matsumoto and Tatjana Paunesku and Woloschak, {Gayle E.} and Tetsuya Ono",

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AU - Ito, Yasuko

AU - Taki, Keiko

AU - Nenoi, Mitsuru

AU - Ichinohe, Kazuaki

AU - Nakamura, Shingo

AU - Tanaka, Satoshi

AU - Oghiso, Yoichi

AU - Tanaka, Kimio

AU - Matsumoto, Tsuneya

AU - Paunesku, Tatjana

AU - Woloschak, Gayle E.

AU - Ono, Tetsuya

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AB - Changes in gene expression profiles in mouse liver induced by long-term low-dose-rate irradiation were examined by microarray analysis. Three groups of male C57BL/6J mice were exposed to whole-body radiation at dose rates of 1720 mGy/day, 0.861.0 mGy/day or 0.0420.050 mGy/day for 401485 days with cumulative doses of approximately 8 Gy, 0.4 Gy or 0.02 Gy, respectively. The gene expression levels in the livers of six animals from each exposure group were compared individually with that of pooled sham-irradiated animals. Some genes revealed a large variation in expression levels among individuals within each group, and the number of genes showing common changes in individuals from each group was limited: 20 and 11 genes showed more than 1.5-fold modulation with 1720 mGy/day and 0.861.0 mGy/day, respectively. Three genes showed more than 1.5-fold modulation even at the lowest dose-rate of 0.040.05 mGy/day. Most of these genes were down-regulated. RT-PCR analysis confirmed the expression profiles of the majority of these genes. The results indicate that a few genes are modulated in response to very low-dose-rate irradiation. The functional analysis suggests that these genes may influence many processes, including obesity and tumorigenesis.

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Gene expression profiles in mouse liver after long-term low-dose-rate irradiation with gamma rays

Abstract

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