Abstract
In vitro cellular senescence of human diploid fibroblast has been a good model for aging research, which shows similar phenotypes to in vivo aging. Gene expression profiling would provide an insight to understand the mechanism of senescence. Using cDNA microarray containing 384 known genes, we compared the expression profiles of three different types of aging models: replicative senescence, fibroblasts from progeria or from elderly donor. Although all of them showed senescence phenotypes, distinct sets of genes were altered in each group. Pairwise plots or cluster analysis of activation fold of gene expression revealed closer relationships between fibroblasts from progeria or from old individual, but not between replicative senescence fibroblasts and either models. Differential expression pattern of several genes were confirmed by RT-PCR. We suggest that the replicative senescence model might behave differently to other types of aging models due to the distinct gene expression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 934-939 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 282 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2001 |
Funding
We thank Dr. Rana Lane for her careful reading this manuscript. This work was partially supported by Seoul National University to W.-Y. Park (No. 800-19990003) and also by Macrogen, Inc. The data will be available at our homepage http://www.macrogen.com/ BBRC_Park.xls. This study was supported in part by the year 2000 BK21 project for Medicine, Dentistry, and Pharmacy to J.-S. Seo.
Keywords
- Aging
- HDF
- Progeria
- Replicative senescence
- cDNA microarray
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology