Gene profile of replicative senescence is different from progeria or elderly donor

Woong Yang Park, Chang Il Hwang, Min Ji Kang, Jin Young Seo, Jin Ho Chung, Yon Su Kim, Jung Hwa Lee, Ho Kim, Kyung A. Kim, Hyung Jin Yoo, Jeong Sun Seo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

In vitro cellular senescence of human diploid fibroblast has been a good model for aging research, which shows similar phenotypes to in vivo aging. Gene expression profiling would provide an insight to understand the mechanism of senescence. Using cDNA microarray containing 384 known genes, we compared the expression profiles of three different types of aging models: replicative senescence, fibroblasts from progeria or from elderly donor. Although all of them showed senescence phenotypes, distinct sets of genes were altered in each group. Pairwise plots or cluster analysis of activation fold of gene expression revealed closer relationships between fibroblasts from progeria or from old individual, but not between replicative senescence fibroblasts and either models. Differential expression pattern of several genes were confirmed by RT-PCR. We suggest that the replicative senescence model might behave differently to other types of aging models due to the distinct gene expression.

Original languageEnglish (US)
Pages (from-to)934-939
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume282
Issue number4
DOIs
StatePublished - 2001

Keywords

  • Aging
  • HDF
  • Progeria
  • Replicative senescence
  • cDNA microarray

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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