Gene regulation with polyvalent siRNA-nanoparticle conjugates

David A. Giljohann, Dwight S. Seferos, Andrew E. Prigodich, Pinal C. Patel, Chad A. Mirkin

Research output: Contribution to journalArticlepeer-review

474 Scopus citations

Abstract

We report the synthesis and characterization of polyvalent RNA-gold nanoparticle conjugates (RNA-Au NPs), nanoparticles that are densely functionalized with synthetic RNA oligonucleotides and designed to function in the RNAi pathway. The particles were rationally designed and synthesized to be free of degrading enzymes, have a high surface loading of siRNA duplexes, and contain an auxiliary passivating agent for increased stability in biological media. The resultant conjugates have a half-life six times longer than that of free dsRNA, readily enter cells without the use of transfection agents, and demonstrate a high gene knockdown capability in a cell model.

Original languageEnglish (US)
Pages (from-to)2072-2073
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number6
DOIs
StatePublished - Feb 18 2009

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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