TY - JOUR
T1 - Gene silencing by double-stranded RNA
AU - Carthew, R. W.
PY - 2001/4/1
Y1 - 2001/4/1
N2 - Eukaryotes silence gene expression in the presence of double-stranded RNA homologous to the silenced gene. Silencing occurs by the targeted degradation of mRNA. Biochemical reactions that recapitulate this phenomenon generate RNA fragments of 21-23 nucleotides from the double-stranded RNA. These stably associate with an RNA endonuclease and probably serve as a discriminator to select mRNAs. Once selected, mRNAs are cleaved at sites 21-23 nucleotides apart. This mechanism, termed RNAi, has functional links to viral defense and silencing phenomena, such as cosuppression. It also functions to repress the hopping of transposable elements.
AB - Eukaryotes silence gene expression in the presence of double-stranded RNA homologous to the silenced gene. Silencing occurs by the targeted degradation of mRNA. Biochemical reactions that recapitulate this phenomenon generate RNA fragments of 21-23 nucleotides from the double-stranded RNA. These stably associate with an RNA endonuclease and probably serve as a discriminator to select mRNAs. Once selected, mRNAs are cleaved at sites 21-23 nucleotides apart. This mechanism, termed RNAi, has functional links to viral defense and silencing phenomena, such as cosuppression. It also functions to repress the hopping of transposable elements.
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U2 - 10.1016/S0955-0674(00)00204-0
DO - 10.1016/S0955-0674(00)00204-0
M3 - Review article
C2 - 11248560
AN - SCOPUS:0035313837
SN - 0955-0674
VL - 13
SP - 244
EP - 248
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
IS - 2
ER -