Gene silencing in the therapy of influenza and other respiratory diseases

Targeting to RNase P by use of external guide sequences (EGS)

David H. Dreyfus, S. Mark Tompkins, Ramsay Fuleihan, Lucy Y. Ghoda

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Respiratory diseases provide an attractive target for gene silencing using small nucleic acids since the respiratory epithelium can be reached by inhalation therapy. Natural surfactant appears to facilitate the uptake and distribution of these types of molecules making aerosolized nucleic acids a possible new class of therapeutics. This article will review the rationale for the use of External Guide Sequence (EGS) in targeting specific mRNA molecules for RNase P-mediated intracellular destruction. Specific destruction of target mRNA results in gene-specific silencing similar to that instigated by siRNA via the RISC complex. The application of EGS molecules specific for influenza genes are discussed as well as the potential for synergy with siRNA. Furthermore, EGS could be adapted to target other respiratory diseases of viral etiology as well as conditions such as asthma.

Original languageEnglish (US)
Pages (from-to)425-432
Number of pages8
JournalBiologics: Targets and Therapy
Volume1
Issue number4
StatePublished - Dec 1 2007

Fingerprint

Ribonuclease P
Gene Silencing
Nucleic Acids
Human Influenza
Small Interfering RNA
Respiratory Therapy
Respiratory Mucosa
Messenger RNA
Virus Diseases
Surface-Active Agents
Asthma
Therapeutics
Genes

ASJC Scopus subject areas

  • Oncology
  • Immunology and Allergy
  • Pharmacology (medical)
  • Gastroenterology
  • Rheumatology

Cite this

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abstract = "Respiratory diseases provide an attractive target for gene silencing using small nucleic acids since the respiratory epithelium can be reached by inhalation therapy. Natural surfactant appears to facilitate the uptake and distribution of these types of molecules making aerosolized nucleic acids a possible new class of therapeutics. This article will review the rationale for the use of External Guide Sequence (EGS) in targeting specific mRNA molecules for RNase P-mediated intracellular destruction. Specific destruction of target mRNA results in gene-specific silencing similar to that instigated by siRNA via the RISC complex. The application of EGS molecules specific for influenza genes are discussed as well as the potential for synergy with siRNA. Furthermore, EGS could be adapted to target other respiratory diseases of viral etiology as well as conditions such as asthma.",
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Gene silencing in the therapy of influenza and other respiratory diseases : Targeting to RNase P by use of external guide sequences (EGS). / Dreyfus, David H.; Tompkins, S. Mark; Fuleihan, Ramsay; Ghoda, Lucy Y.

In: Biologics: Targets and Therapy, Vol. 1, No. 4, 01.12.2007, p. 425-432.

Research output: Contribution to journalReview article

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T2 - Targeting to RNase P by use of external guide sequences (EGS)

AU - Dreyfus, David H.

AU - Tompkins, S. Mark

AU - Fuleihan, Ramsay

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