Gene-specific characterization of human histone H2B by electron capture dissociation

Nertila Siuti; Michael J. Roth; Craig A. Mizzen; Neil L. Kelleher; James J. Pesavento

doi:10.1021/pr050268v

Gene-specific characterization of human histone H2B by electron capture dissociation

Nertila Siuti, Michael J. Roth, Craig A. Mizzen, Neil L. Kelleher^*, James J. Pesavento

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

The basis set of protein forms expressed by human cells from the H2B gene family was determined by Top Down Mass Spectrometry. Using Electron Capture Dissociation for MS/MS of H2B isoforms, direct evidence for the expression of unmodified H2B.Q, H2B.A, H2B.K/T, H2B.J, H2B.E, H2B.B, H2B.F, and monoacetylated H2B.A was obtained from asynchronous HeLa cells. H2B.A was the most abundant form, with the overall expression profile not changing significantly in cells arrested in mitosis by colchicine or during mid-S, mid-G2, G2/M, and mid-G1 phases of the cell cycle. Modest hyperacetylation of H2B family members was observed after sodium butyrate treatment.

Original language	English (US)
Pages (from-to)	233-239
Number of pages	7
Journal	Journal of Proteome Research
Volume	5
Issue number	2
DOIs	https://doi.org/10.1021/pr050268v
State	Published - Feb 2006

Keywords

Chromatin
Electron capture dissociation
Histone
Post-translational modifications
Top down mass spectrometry

ASJC Scopus subject areas

Biochemistry
General Chemistry

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10.1021/pr050268v

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title = "Gene-specific characterization of human histone H2B by electron capture dissociation",

abstract = "The basis set of protein forms expressed by human cells from the H2B gene family was determined by Top Down Mass Spectrometry. Using Electron Capture Dissociation for MS/MS of H2B isoforms, direct evidence for the expression of unmodified H2B.Q, H2B.A, H2B.K/T, H2B.J, H2B.E, H2B.B, H2B.F, and monoacetylated H2B.A was obtained from asynchronous HeLa cells. H2B.A was the most abundant form, with the overall expression profile not changing significantly in cells arrested in mitosis by colchicine or during mid-S, mid-G2, G2/M, and mid-G1 phases of the cell cycle. Modest hyperacetylation of H2B family members was observed after sodium butyrate treatment.",

keywords = "Chromatin, Electron capture dissociation, Histone, Post-translational modifications, Top down mass spectrometry",

author = "Nertila Siuti and Roth, {Michael J.} and Mizzen, {Craig A.} and Kelleher, {Neil L.} and Pesavento, {James J.}",

year = "2006",

month = feb,

doi = "10.1021/pr050268v",

language = "English (US)",

volume = "5",

pages = "233--239",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Gene-specific characterization of human histone H2B by electron capture dissociation

AU - Siuti, Nertila

AU - Roth, Michael J.

AU - Mizzen, Craig A.

AU - Kelleher, Neil L.

AU - Pesavento, James J.

PY - 2006/2

Y1 - 2006/2

N2 - The basis set of protein forms expressed by human cells from the H2B gene family was determined by Top Down Mass Spectrometry. Using Electron Capture Dissociation for MS/MS of H2B isoforms, direct evidence for the expression of unmodified H2B.Q, H2B.A, H2B.K/T, H2B.J, H2B.E, H2B.B, H2B.F, and monoacetylated H2B.A was obtained from asynchronous HeLa cells. H2B.A was the most abundant form, with the overall expression profile not changing significantly in cells arrested in mitosis by colchicine or during mid-S, mid-G2, G2/M, and mid-G1 phases of the cell cycle. Modest hyperacetylation of H2B family members was observed after sodium butyrate treatment.

AB - The basis set of protein forms expressed by human cells from the H2B gene family was determined by Top Down Mass Spectrometry. Using Electron Capture Dissociation for MS/MS of H2B isoforms, direct evidence for the expression of unmodified H2B.Q, H2B.A, H2B.K/T, H2B.J, H2B.E, H2B.B, H2B.F, and monoacetylated H2B.A was obtained from asynchronous HeLa cells. H2B.A was the most abundant form, with the overall expression profile not changing significantly in cells arrested in mitosis by colchicine or during mid-S, mid-G2, G2/M, and mid-G1 phases of the cell cycle. Modest hyperacetylation of H2B family members was observed after sodium butyrate treatment.

KW - Chromatin

KW - Electron capture dissociation

KW - Histone

KW - Post-translational modifications

KW - Top down mass spectrometry

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JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 2

ER -

Gene-specific characterization of human histone H2B by electron capture dissociation

Abstract

Keywords

ASJC Scopus subject areas

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