Generating a Cost-Effective, Weekend-Free Chemically Defined Human Induced Pluripotent Stem Cell (hiPSC) Culture Medium

Hananeh Fonoudi; Davi M. Lyra-Leite; Hoor A. Javed; Paul W. Burridge

doi:10.1002/cpsc.110

Generating a Cost-Effective, Weekend-Free Chemically Defined Human Induced Pluripotent Stem Cell (hiPSC) Culture Medium

Hananeh Fonoudi, Davi M. Lyra-Leite, Hoor A. Javed, Paul W. Burridge^*

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We have previously developed a cost-effective chemically defined medium formula for weekend-free culture of human induced pluripotent stem cells (hiPSCs), costing ∼3% of the price of commercial medium. This medium, which we termed B8, is specifically optimized for robust and fast growth of hiPSCs and for a weekend-free medium change regimen. We demonstrated that this medium is suitable for reprogramming of somatic cells into hiPSCs and for differentiation into a variety of lineages. Here, we provide a protocol for simple generation of the most cost-effective variant of this medium, along with a protocol for making Matrigel-coated plates and culturing, passaging, cryopreserving, and thawing hiPSCs.

Original language	English (US)
Article number	e110
Journal	Current Protocols in Stem Cell Biology
Volume	53
Issue number	1
DOIs	https://doi.org/10.1002/cpsc.110
State	Published - Jun 1 2020

Funding

This work was supported by NIH NCI grant R01 CA220002, American Heart Association Transformational Project Award 18TPA34230105, and the Foundation Leducq (P.W.B.).

Keywords

chemically defined
cost-effective
culture medium
human induced pluripotent stem cells
weekend-free

ASJC Scopus subject areas

Cell Biology
Developmental Biology

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10.1002/cpsc.110

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AU - Burridge, Paul W.

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Generating a Cost-Effective, Weekend-Free Chemically Defined Human Induced Pluripotent Stem Cell (hiPSC) Culture Medium

Abstract

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Keywords

ASJC Scopus subject areas

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