Generation and identification of tumor-evoked regulatory B cells

Arya Biragyn, Catalina Lee Chang, Monica Bodogai

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

The involvement of Bregs in cancer remains poorly understood despite their well-documented regulation of responses to the self and protection from harmful autoimmunity. We recently discovered a unique regulatory B cell subset evoked by breast cancer to mediate protection of metastasizing cancer cells. These results together with the wealth of findings of the last 40 years on B cells in tumorigenesis suggest the existence of additional cancer Bregs modulating anticancer responses. To facilitate the search for them, here we provide our detailed protocol for the characterization and generation of tumor-evoked regulatory B cells. Wherever applicable, we also discuss nuances and uniqueness of a Breg study in cancer to warn potential pitfalls.

Original languageEnglish (US)
Title of host publicationRegulatory B Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages271-289
Number of pages19
ISBN (Print)9781493911608
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1190
ISSN (Print)1064-3745

Keywords

  • 4T1 breast cancer
  • Metastasis
  • TARC-PE38
  • Tregs
  • tBregs

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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