Generation of a mouse for conditional excision of progesterone receptor

Minako K. Hashimoto-Partyka, John P. Lydon, M. Luisa Iruela-Arispe*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The progesterone receptor (PR) is required for several aspects of mammalian female reproduction. PR null mice have overlapping defects that preclude an understanding of its multiple functions in ovulation, pregnancy, mammary gland biology, and sexual behavior. We have generated a PR conditional excision (PRCE) allele in which IoxP sites flank exon 1. Homozygous PRCE females are fertile and appear to be functionally normal. Global cre mediated excision of the floxed exon 1 using EIIa-cre mice resulted in systemic loss of exon 1 and PR protein. Female mice homozygous for this null allele were sterile, as expected for PR knockout (PRKO) females. Conditional loss of PR will facilitate investigation of the spatial and temporal roles of PR in both normal development and disease.

Original languageEnglish (US)
Pages (from-to)391-395
Number of pages5
JournalGenesis
Volume44
Issue number8
DOIs
StatePublished - Aug 1 2006

Keywords

  • Gene targeting
  • Mammary gland
  • Ovary
  • Steroid receptors
  • Uterus

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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