TY - JOUR
T1 - Generation of an inducible mouse ES cell lines deficient for Argonaute proteins.
AU - Su, Hong
AU - Wang, Xiaozhong
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2011
Y1 - 2011
N2 - Argonautes (Agos) are core effectors of RNA silencing. In several nonmammalian organisms, multiple Agos are known to exhibit specialized functions for distinct RNA silencing pathway. Mammals have four closely related Agos. To examine the functions of mammalian Agos in the microRNA silencing pathway, we generated mouse embryonic stem (ES) cells that are nullizygous for all Agos. This chapter describes a variety of techniques including BAC recombineering, gene targeting, and inducible Cre-loxP recombination, used to generate inducible Ago knock-out ES cells. The Ago-deficient ES cells provide an important tool for the study of mammalian RNA silencing.
AB - Argonautes (Agos) are core effectors of RNA silencing. In several nonmammalian organisms, multiple Agos are known to exhibit specialized functions for distinct RNA silencing pathway. Mammals have four closely related Agos. To examine the functions of mammalian Agos in the microRNA silencing pathway, we generated mouse embryonic stem (ES) cells that are nullizygous for all Agos. This chapter describes a variety of techniques including BAC recombineering, gene targeting, and inducible Cre-loxP recombination, used to generate inducible Ago knock-out ES cells. The Ago-deficient ES cells provide an important tool for the study of mammalian RNA silencing.
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U2 - 10.1007/978-1-61779-046-1_19
DO - 10.1007/978-1-61779-046-1_19
M3 - Article
C2 - 21528461
AN - SCOPUS:80051576113
VL - 725
SP - 295
EP - 313
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -