Generation of conditional alleles for Foxc1 and Foxc2 in mice

Amy Sasman, Carey Nassano-Miller, Kyoo Seok Shim, Hyun Young Koo, Ting Liu, Kathryn M. Schultz, Meredith Millay, Atsushi Nanano, Myengmo Kang, Takashi Suzuki, Tsutomu Kume*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The Forkhead box transcription factors, Foxc1 and Foxc2, are crucial for development of the eye, cardiovascular network, and other physiological systems, but their cell-type specific and postdevelopmental functions are unknown, in part because conventional (i.e., whole-organism) homozygous-null mutations of either factor result in perinatal death. Here, we describe the generation of mice with conditional-null Foxc1flox and Foxc2flox mutations that are induced via Cre-mediated recombination. Mice homozygous for the unrecombined alleles are viable and fertile, indicating that the conditional alleles retain their wild-type function. The embryos of Foxc1flox or Foxc2flox mice crossed with Cre-deleter mice that are homozygous for the recombined allele (i.e., Foxc1Δ/Δ or Foxc2Δ/Δ embryos) lack expression of the corresponding gene and show the same developmental defects observed in conventional homozygous mutant embryos. We expect these conditional mutations to enable characterization of the cell-type specific functions of Foxc1 and Foxc2 in development, disease, and adult animals.

Original languageEnglish (US)
Pages (from-to)766-774
Number of pages9
JournalGenesis
Volume50
Issue number10
DOIs
StatePublished - Oct 2012

Keywords

  • Conditional knockout
  • Cre recombination
  • Foxc1
  • Foxc2
  • Gene targeting

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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