Generation of conditional alleles for Foxc1 and Foxc2 in mice

Amy Sasman, Carey Nassano-Miller, Kyoo Seok Shim, Hyun Young Koo, Ting Liu, Kathryn M. Schultz, Meredith Millay, Atsushi Nanano, Myengmo Kang, Takashi Suzuki, Tsutomu Kume*

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The Forkhead box transcription factors, Foxc1 and Foxc2, are crucial for development of the eye, cardiovascular network, and other physiological systems, but their cell-type specific and postdevelopmental functions are unknown, in part because conventional (i.e., whole-organism) homozygous-null mutations of either factor result in perinatal death. Here, we describe the generation of mice with conditional-null Foxc1flox and Foxc2flox mutations that are induced via Cre-mediated recombination. Mice homozygous for the unrecombined alleles are viable and fertile, indicating that the conditional alleles retain their wild-type function. The embryos of Foxc1flox or Foxc2flox mice crossed with Cre-deleter mice that are homozygous for the recombined allele (i.e., Foxc1Δ/Δ or Foxc2Δ/Δ embryos) lack expression of the corresponding gene and show the same developmental defects observed in conventional homozygous mutant embryos. We expect these conditional mutations to enable characterization of the cell-type specific functions of Foxc1 and Foxc2 in development, disease, and adult animals.

Original languageEnglish (US)
Pages (from-to)766-774
Number of pages9
JournalGenesis
Volume50
Issue number10
DOIs
StatePublished - Oct 1 2012

    Fingerprint

Keywords

  • Conditional knockout
  • Cre recombination
  • Foxc1
  • Foxc2
  • Gene targeting

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Sasman, A., Nassano-Miller, C., Shim, K. S., Koo, H. Y., Liu, T., Schultz, K. M., Millay, M., Nanano, A., Kang, M., Suzuki, T., & Kume, T. (2012). Generation of conditional alleles for Foxc1 and Foxc2 in mice. Genesis, 50(10), 766-774. https://doi.org/10.1002/dvg.22036