Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Tomoyo Sawada; Kynon J.M. Benjamin; Anna C. Brandtjen; Ethan Tietze; Samuel J. Allen; Apuã C.M. Paquola; Joel E. Kleinman; Thomas M. Hyde; Jennifer A. Erwin

doi:10.1016/j.scr.2020.101806

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Tomoyo Sawada^*, Kynon J.M. Benjamin, Anna C. Brandtjen, Ethan Tietze, Samuel J. Allen, Apuã C.M. Paquola, Joel E. Kleinman, Thomas M. Hyde, Jennifer A. Erwin

^*Corresponding author for this work

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

Original language	English (US)
Article number	101806
Journal	Stem Cell Research
Volume	46
DOIs	https://doi.org/10.1016/j.scr.2020.101806
State	Published - Jul 2020

Funding

Supported by funding from the Lieber Institute for Brain Development, T32 funding (T32MH015330) from NIH to K.J.M.B. and a NARSAD Young Investigator Grant from the Brain & Behavior Research Foundation to J.A.E.

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1016/j.scr.2020.101806

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Sawada, T., Benjamin, K. J. M., Brandtjen, A. C., Tietze, E., Allen, S. J., Paquola, A. C. M., Kleinman, J. E., Hyde, T. M., & Erwin, J. A. (2020). Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium. Stem Cell Research, 46, Article 101806. https://doi.org/10.1016/j.scr.2020.101806

@article{23b87eb28adc41d7ac6ffc4fc3adbf42,

title = "Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.",

abstract = "In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.",

author = "Tomoyo Sawada and Benjamin, \{Kynon J.M.\} and Brandtjen, \{Anna C.\} and Ethan Tietze and Allen, \{Samuel J.\} and Paquola, \{Apu{\~a} C.M.\} and Kleinman, \{Joel E.\} and Hyde, \{Thomas M.\} and Erwin, \{Jennifer A.\}",

note = "Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2020",

month = jul,

doi = "10.1016/j.scr.2020.101806",

language = "English (US)",

volume = "46",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

Sawada, T, Benjamin, KJM, Brandtjen, AC, Tietze, E, Allen, SJ, Paquola, ACM, Kleinman, JE, Hyde, TM & Erwin, JA 2020, 'Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.', Stem Cell Research, vol. 46, 101806. https://doi.org/10.1016/j.scr.2020.101806

Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium. / Sawada, Tomoyo; Benjamin, Kynon J.M.; Brandtjen, Anna C. et al.
In: Stem Cell Research, Vol. 46, 101806, 07.2020.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

AU - Sawada, Tomoyo

AU - Benjamin, Kynon J.M.

AU - Brandtjen, Anna C.

AU - Tietze, Ethan

AU - Allen, Samuel J.

AU - Paquola, Apuã C.M.

AU - Kleinman, Joel E.

AU - Hyde, Thomas M.

AU - Erwin, Jennifer A.

PY - 2020/7

Y1 - 2020/7

N2 - In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

AB - In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.

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Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and brain-region-specific transcriptomic data available through the BrainSEQ consortium.

Abstract

Funding

ASJC Scopus subject areas

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