Generation of genetically modified mice using the CRISPR-Cas9 genome-editing system

Jorge Henao-Mejia*, Adam Williams, Anthony Rongvaux, Judith Stein, Cynthia Hughes, Richard A. Flavell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Genetically modified mice are extremely valuable tools for studying gene function and human diseases. Although the generation of mice with specific genetic modifications through traditional methods using homologous recombination in embryonic stem cells has been invaluable in the last two decades, it is an extremely costly, time-consuming, and, in some cases, uncertain technology. The recently described CRISPR-Cas9 genome-editing technology significantly reduces the time and the cost that are required to generate genetically engineered mice, allowing scientists to test more precise and bold hypotheses in vivo. Using this revolutionary methodology we have generated more than 100 novel genetically engineered mouse strains. In the current protocol, we describe in detail the optimal conditions to generate mice carrying point mutations, chromosomal deletions, conditional alleles, fusion tags, or endogenous reporters.

Original languageEnglish (US)
Pages (from-to)150-159
Number of pages10
JournalCold Spring Harbor Protocols
Issue number2
StatePublished - Feb 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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