Generation of high-spin iron(I) in a protein environment using cryoreduction

Roman M. Davydov, Matthew P. McLaughlin, Eckhard Bill, Brian M. Hoffman*, Patrick L. Holland

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

High-spin Fe1+ sites are potentially important in iron-sulfur proteins but are rare in synthetic compounds and unknown in metalloproteins. Here, we demonstrate a spectroscopically characterized example of high-spin non-heme Fe1+ in a protein environment. Cryoreduction of Fe 2+-substituted azurin at 77 K with 60Co γ radiation generates a new species with a S = 3/2 (high-spin) Fe 1+ center having D > 0 and E/D ∼ 0.25. This transient species is stable in a glycerol-water glass only up to ∼170 K. A combination of electron paramagnetic resonance and Mössbauer spectroscopies provides a powerful means of identifying a transient high-spin Fe1+ site in a protein scaffold.

Original languageEnglish (US)
Pages (from-to)7323-7325
Number of pages3
JournalInorganic chemistry
Volume52
Issue number13
DOIs
StatePublished - Jul 1 2013

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry

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