Generation of human iPSCs from urine derived cells of a non-affected control subject

Muhammad Zeeshan Afzal, Melanie Gartz, Ekaterina A. Klyachko, Sadiya Sana Khan, Sanjiv J. Shah, Sweta Gupta, Amy D. Shapiro, Douglas E. Vaughan, Jennifer L. Strande*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.

Original languageEnglish (US)
Pages (from-to)33-36
Number of pages4
JournalStem Cell Research
Volume18
DOIs
StatePublished - Jan 2017

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Generation of human iPSCs from urine derived cells of a non-affected control subject'. Together they form a unique fingerprint.

Cite this