Generation of human iPSCs from urine derived cells of a non-affected control subject

Muhammad Zeeshan Afzal; Melanie Gartz; Ekaterina A. Klyachko; Sadiya Sana Khan; Sanjiv J. Shah; Sweta Gupta; Amy D. Shapiro; Douglas E. Vaughan; Jennifer L. Strande

doi:10.1016/j.scr.2016.12.008

Generation of human iPSCs from urine derived cells of a non-affected control subject

Muhammad Zeeshan Afzal, Melanie Gartz, Ekaterina A. Klyachko, Sadiya Sana Khan, Sanjiv J. Shah, Sweta Gupta, Amy D. Shapiro, Douglas E. Vaughan, Jennifer L. Strande^*

^*Corresponding author for this work

Medicine, Cardiology Division

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.

Original language	English (US)
Pages (from-to)	33-36
Number of pages	4
Journal	Stem Cell Research
Volume	18
DOIs	https://doi.org/10.1016/j.scr.2016.12.008
State	Published - Jan 2017

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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@article{e88142345fba466fa02d5e4229be285d,

title = "Generation of human iPSCs from urine derived cells of a non-affected control subject",

abstract = "We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.",

author = "Afzal, {Muhammad Zeeshan} and Melanie Gartz and Klyachko, {Ekaterina A.} and Khan, {Sadiya Sana} and Shah, {Sanjiv J.} and Sweta Gupta and Shapiro, {Amy D.} and Vaughan, {Douglas E.} and Strande, {Jennifer L.}",

note = "Funding Information: This work was supported by the National Institutes of Health K08 grant number HL111148 (J.L.S.) and R01 grant number HL051387 (D.E.V.). We are especially indebted to patients and their families for participating in this study and donating samples. ",

year = "2017",

month = jan,

doi = "10.1016/j.scr.2016.12.008",

language = "English (US)",

volume = "18",

pages = "33--36",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

Generation of human iPSCs from urine derived cells of a non-affected control subject. / Afzal, Muhammad Zeeshan; Gartz, Melanie; Klyachko, Ekaterina A.; Khan, Sadiya Sana ; Shah, Sanjiv J.; Gupta, Sweta; Shapiro, Amy D.; Vaughan, Douglas E.; Strande, Jennifer L.

In: Stem Cell Research, Vol. 18, 01.2017, p. 33-36.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Afzal, Muhammad Zeeshan

AU - Gartz, Melanie

AU - Klyachko, Ekaterina A.

AU - Khan, Sadiya Sana

AU - Shah, Sanjiv J.

AU - Gupta, Sweta

AU - Shapiro, Amy D.

AU - Vaughan, Douglas E.

AU - Strande, Jennifer L.

N1 - Funding Information: This work was supported by the National Institutes of Health K08 grant number HL111148 (J.L.S.) and R01 grant number HL051387 (D.E.V.). We are especially indebted to patients and their families for participating in this study and donating samples.

PY - 2017/1

Y1 - 2017/1

N2 - We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.

AB - We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.

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