Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

Muhammad Zeeshan Afzal, Melanie Gartz, Ekaterina A. Klyachko, Sadiya Sana Khan, Sanjiv J. Shah, Sweta Gupta, Amy D. Shapiro, Douglas E. Vaughan, Jennifer L. Strande*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.

Original languageEnglish (US)
Pages (from-to)657-660
Number of pages4
JournalStem Cell Research
Volume17
Issue number3
DOIs
StatePublished - Nov 2016

Funding

This work was supported by the National Institutes of Health K08 grant number HL111148 (J.L.S.) and R01HL051387 (D.E.V.). We are especially indebted to patients and their families for participating in this study and donating samples.

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

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