Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

Muhammad Zeeshan Afzal, Melanie Gartz, Ekaterina A. Klyachko, Sadiya Sana Khan, Sanjiv J. Shah, Sweta Gupta, Amy D. Shapiro, Douglas E. Vaughan, Jennifer L. Strande*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.

Original languageEnglish (US)
Pages (from-to)657-660
Number of pages4
JournalStem Cell Research
Volume17
Issue number3
DOIs
StatePublished - Nov 2016

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation'. Together they form a unique fingerprint.

Cite this