Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

Muhammad Zeeshan Afzal; Melanie Gartz; Ekaterina A. Klyachko; Sadiya Sana Khan; Sanjiv J. Shah; Sweta Gupta; Amy D. Shapiro; Douglas E. Vaughan; Jennifer L. Strande

doi:10.1016/j.scr.2016.11.010

Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

Muhammad Zeeshan Afzal, Melanie Gartz, Ekaterina A. Klyachko, Sadiya Sana Khan, Sanjiv J. Shah, Sweta Gupta, Amy D. Shapiro, Douglas E. Vaughan, Jennifer L. Strande^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.

Original language	English (US)
Pages (from-to)	657-660
Number of pages	4
Journal	Stem Cell Research
Volume	17
Issue number	3
DOIs	https://doi.org/10.1016/j.scr.2016.11.010
State	Published - Nov 2016

Funding

This work was supported by the National Institutes of Health K08 grant number HL111148 (J.L.S.) and R01HL051387 (D.E.V.). We are especially indebted to patients and their families for participating in this study and donating samples.

ASJC Scopus subject areas

Cell Biology
Developmental Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation",

abstract = "We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.",

author = "Afzal, {Muhammad Zeeshan} and Melanie Gartz and Klyachko, {Ekaterina A.} and Khan, {Sadiya Sana} and Shah, {Sanjiv J.} and Sweta Gupta and Shapiro, {Amy D.} and Vaughan, {Douglas E.} and Strande, {Jennifer L.}",

note = "Publisher Copyright: {\textcopyright} 2016 Michael Boutros, German Cancer Research Center, Heidelberg, Germany",

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doi = "10.1016/j.scr.2016.11.010",

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AU - Afzal, Muhammad Zeeshan

AU - Gartz, Melanie

AU - Klyachko, Ekaterina A.

AU - Khan, Sadiya Sana

AU - Shah, Sanjiv J.

AU - Gupta, Sweta

AU - Shapiro, Amy D.

AU - Vaughan, Douglas E.

AU - Strande, Jennifer L.

PY - 2016/11

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AB - We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.

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Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

Abstract

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ASJC Scopus subject areas

UN SDGs

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