Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Haiyun Pei; Hsu Yuan Fu; Dong Seong Cho; Wei Shou Hu; Haiyun Pei; Dong Seong Cho; Timothy D. O'Brien; James Dutton; Wei Shou Hu; Haiyun Pei; Hiroyuki Hirai; Catherine M. Verfaillie; Timothy D. O'Brien

doi:10.1016/j.scr.2017.04.010

Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Haiyun Pei, Hsu Yuan Fu, Dong Seong Cho, Wei Shou Hu, Haiyun Pei, Dong Seong Cho, Timothy D. O'Brien, James Dutton, Wei Shou Hu, Haiyun Pei, Hiroyuki Hirai, Catherine M. Verfaillie, Timothy D. O'Brien

Pediatrics

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M₃O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.

Original language	English (US)
Pages (from-to)	132-136
Number of pages	5
Journal	Stem Cell Research
Volume	21
DOIs	https://doi.org/10.1016/j.scr.2017.04.010
State	Published - May 1 2017

Funding

We thank Dr. Kyoungho Lee for his pLOVE-M3O vector construction. Histological analyses were performed at the University of Minnesota Comparative Pathology Shared Resource. The cytogenetic analyses were performed in the Cytogenomics Shared Resource at the University of Minnesota with support from the comprehensive Masonic Cancer Center NIH Grant #P30 CA077598.

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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title = "Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts",

abstract = "We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.",

author = "Haiyun Pei and Fu, {Hsu Yuan} and Cho, {Dong Seong} and Hu, {Wei Shou} and Haiyun Pei and Cho, {Dong Seong} and O'Brien, {Timothy D.} and James Dutton and Hu, {Wei Shou} and Haiyun Pei and Hiroyuki Hirai and Verfaillie, {Catherine M.} and O'Brien, {Timothy D.}",

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doi = "10.1016/j.scr.2017.04.010",

language = "English (US)",

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AU - Pei, Haiyun

AU - Fu, Hsu Yuan

AU - Cho, Dong Seong

AU - Hu, Wei Shou

AU - Pei, Haiyun

AU - Cho, Dong Seong

AU - O'Brien, Timothy D.

AU - Dutton, James

AU - Hu, Wei Shou

AU - Pei, Haiyun

AU - Hirai, Hiroyuki

AU - Verfaillie, Catherine M.

AU - O'Brien, Timothy D.

PY - 2017/5/1

Y1 - 2017/5/1

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AB - We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.

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Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Abstract

Funding

ASJC Scopus subject areas

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