Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Haiyun Pei, Hsu Yuan Fu, Dong Seong Cho, Wei Shou Hu, Haiyun Pei, Dong Seong Cho, Timothy D. O'Brien, James Dutton, Wei Shou Hu, Haiyun Pei, Hiroyuki Hirai, Catherine M. Verfaillie, Timothy D. O'Brien

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.

Original languageEnglish (US)
Pages (from-to)132-136
Number of pages5
JournalStem Cell Research
Volume21
DOIs
StatePublished - May 1 2017

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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