Generation of Phosphopeptide-Specific T Cell Lines as Tools for Melanoma Immunotherapy

Rebecca C. Obeng*, Angela L. Ambakhutwala

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The importance of tumor-associated antigen-specific T cells in the effective control of cancer has been highlighted by recent advances in cancer immunotherapies that target the programmed cell death-1 (PD-1) pathway or that utilize modified T cell receptors. Phosphopeptide-specific T cells are of interest because they recognize a new class of tumor antigens that are derived from proteins relevant for cancer development and growth. These T cell lines or their antigen receptors can be used in combination with other forms of therapy to improve the immune response and survival of cancer patients. We describe here a protocol for the generation of human and transgenic murine phosphopeptide-specific T cells lines as tools for investigating T cell reactivity against melanoma phosphoantigens displayed by HLA-A*0201.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages655-670
Number of pages16
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2265
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Antigens
  • Immunotherapy
  • Phosphopeptides
  • T cell lines
  • T cell therapy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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