Abstract
Limestone karsts of Southeast Asia can harbor high levels of endemism, but are highly fragmented, increasingly threatened, and their biodiversity is often poorly studied. This is true of the Padawan Limestone Area of Sarawak, Malaysia, home to the endemic Artocarpus annulatus, the closest known wild relative of two important and underutilized fruit tree crops, jackfruit (A. heterophyllus) and cempedak (A. integer). Identifying and conserving crop wild relatives is critical for the conservation of crop genetic diversity and breeding. In 2016 and 2017, five A. annulatus populations were located, and leaf material, locality information, and demographic data were collected. Microsatellite markers were used to assess genetic diversity and structure among populations, and to compare levels of genetic diversity to closely related congeneric species. Results indicate no evidence of inbreeding in A. annulatus, and there is no genetic structure among the five populations. However, diversity measures trended lower in seedlings compared to mature trees, suggesting allelic diversity may be under threat in the youngest generation of plants. Also, genetic diversity is lower in A. annulatus compared to closely related congeners. The present study provides a baseline estimate of A. annulatus genetic diversity that can be used for comparison in future studies and to other species in the unique limestone karst ecosystems. Considerations for in situ and ex situ conservation approaches are discussed.
Original language | English (US) |
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Article number | e9897 |
Journal | PeerJ |
Volume | 8 |
DOIs | |
State | Published - 2020 |
Funding
This research was supported by Northwestern University's Undergraduate Research Grant program, the Mohamed bin Zayed Species Conservation Fund (project number 152511619), and the National Science Foundation (DBI award number 1711391). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Keywords
- Biodiversity conservation
- Endemic species
- Habitat fragmentation
- Karst limestone
- Malaysia
- Microsatellites
- Plant genetic resources
- Sarawak
ASJC Scopus subject areas
- General Agricultural and Biological Sciences
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience