Genetic evidence for functional redundancy of platelet/endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions

Gordon S. Duncan, David P. Andrew, Hiroaki Takimoto, Stephen A. Kaufman, Hiroki Yoshida, Jason Spellberg, José Luis De La Pompa, Andrew Elia, Andrew Wakeham, Barbara Karan-Tamir, William A. Muller, Giorgio Senaldi, Mark M. Zukowski, Tak W. Mak*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

353 Scopus citations

Abstract

Platelet/endothelial cell adhesion molecule-1 (PECAM-1; CD31), a member of the Ig superfamily, is expressed strongly at endothelial cell-cell junctions, on platelets, and on most leukocytes. CD31 has been postulated to play a role in vasculogenesis and angiogenesis, and has been implicated as a key mediator of the transendothelial migration of leukocytes. To further define the physiologic role of CD31, we used targeted gene disruption of the CD31 gene in embryonic stem cells to generate CD31-deficient mice. CD31- deficient mice (CD31KO) are viable and born at the expected Mendelian frequency, remain healthy, and exhibit no obvious vascular developmental defects. In response to inflammatory challenge, polymorphonuclear leukocytes of CD31KO mice are arrested between the vascular endothelium and the basement membrane of inflammatory site mesenteric microvessels, confirming a role for CD31 in the migration of neutrophils through the subendothelial extracellular matrix. Normal numbers of leukocytes are recovered from inflammatory sites in CD31KO mice, however, suggesting that the defect in leukocyte migration across basal lamina observed in the absence of CD31 may be compensated for by the use of other adhesion molecules, or possibly an increased rate of migration. Homing of T lymphocytes in vivo is normal, and CD31KO mice are able to mount a cutaneous hypersensitivity response normally. In addition, CD31-mediated homophilic adhesion does not appear to play a role in platelet aggregation in vitro. This study provides genetic evidence that CD31 is involved in transbasement membrane migration, but does not play an obligatory role in either vascular development or leukocyte migration.

Original languageEnglish (US)
Pages (from-to)3022-3030
Number of pages9
JournalJournal of Immunology
Volume162
Issue number5
StatePublished - Mar 1 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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