Genetic interactions between chromosomes 11 and 18 contribute to airway hyperresponsiveness in mice

Caroline M. Ferreira, James L. Chen, Jianrong Li, Kazuhiro Shimomura, Xinan Yang, Yves A. Lussier, Lawrence H. Pinto, Julian Solway*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


We used two-dimensional quantitative trait locus analysis to identify interacting genetic loci that contribute to the native airway constrictor hyperresponsiveness to methacholine that characterizes A/J mice, relative to C57BL/6J mice. We quantified airway responsiveness to intravenous methacholine boluses in eighty-eight (C57BL/6J X A/J) F 2 and twenty-seven (A/J X C57BL/6J) F 2 mice as well as ten A/J mice and six C57BL/6J mice; all studies were performed in male mice. Mice were genotyped at 384 SNP markers, and from these data two-QTL analyses disclosed one pair of interacting loci on chromosomes 11 and 18; the homozygous A/J genotype at each locus constituted the genetic interaction linked to the hyperresponsive A/J phenotype. Bioinformatic network analysis of potential interactions among proteins encoded by genes in the linked regions disclosed two high priority subnetworks - Myl7, Rock1, Limk2; and Npc1, Npc1l1. Evidence in the literature supports the possibility that either or both networks could contribute to the regulation of airway constrictor responsiveness. Together, these results should stimulate evaluation of the genetic contribution of these networks in the regulation of airway responsiveness in humans.

Original languageEnglish (US)
Article numbere29579
JournalPloS one
Issue number1
StatePublished - Jan 10 2012

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General
  • General Biochemistry, Genetics and Molecular Biology


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