Genetic modification and screening in rat using haploid embryonic stem cells

Wei Li; Xin Li; Tianda Li; Ming Gui Jiang; Haifeng Wan; Guan Zheng Luo; Chunjing Feng; Xiaolong Cui; Fei Teng; Yan Yuan; Quan Zhou; Qi Gu; Ling Shuai; Jiahao Sha; Yamei Xiao; Liu Wang; Zhonghua Liu; Xiu Jie Wang; Xiao Yang Zhao; Qi Zhou

doi:10.1016/j.stem.2013.11.016

Genetic modification and screening in rat using haploid embryonic stem cells

Wei Li, Xin Li, Tianda Li, Ming Gui Jiang, Haifeng Wan, Guan Zheng Luo, Chunjing Feng, Xiaolong Cui, Fei Teng, Yan Yuan, Quan Zhou^*, Qi Gu, Ling Shuai, Jiahao Sha, Yamei Xiao, Liu Wang, Zhonghua Liu, Xiu Jie Wang, Xiao Yang Zhao, Qi Zhou^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of rat androgenetic haploid embryonic stem cells (RahESCs) as a tool to facilitate such studies. Our approach is based on removal of the maternal pronucleus from zygotes to generate androgenetic embryos followed by derivation of ESCs. The resulting RahESCs have 21 chromosomes, express pluripotency markers, differentiate into three germ layer cells, and contribute to the germline. Homozygous mutations can be introduced by both large-scale gene trapping and precise gene targeting via homologous recombination or the CRISPR-Cas system. RahESCs can also produce fertile rats after intracytoplasmic injection into oocytes and are therefore able to transmit genetic modifications to offspring. Overall, RahESCs represent a practical tool for functional genetic studies and production of transgenic lines in rat.

Original language	English (US)
Pages (from-to)	404-414
Number of pages	11
Journal	Cell stem cell
Volume	14
Issue number	3
DOIs	https://doi.org/10.1016/j.stem.2013.11.016
State	Published - Mar 6 2014
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

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10.1016/j.stem.2013.11.016

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title = "Genetic modification and screening in rat using haploid embryonic stem cells",

abstract = "The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of rat androgenetic haploid embryonic stem cells (RahESCs) as a tool to facilitate such studies. Our approach is based on removal of the maternal pronucleus from zygotes to generate androgenetic embryos followed by derivation of ESCs. The resulting RahESCs have 21 chromosomes, express pluripotency markers, differentiate into three germ layer cells, and contribute to the germline. Homozygous mutations can be introduced by both large-scale gene trapping and precise gene targeting via homologous recombination or the CRISPR-Cas system. RahESCs can also produce fertile rats after intracytoplasmic injection into oocytes and are therefore able to transmit genetic modifications to offspring. Overall, RahESCs represent a practical tool for functional genetic studies and production of transgenic lines in rat.",

author = "Wei Li and Xin Li and Tianda Li and Jiang, {Ming Gui} and Haifeng Wan and Luo, {Guan Zheng} and Chunjing Feng and Xiaolong Cui and Fei Teng and Yan Yuan and Quan Zhou and Qi Gu and Ling Shuai and Jiahao Sha and Yamei Xiao and Liu Wang and Zhonghua Liu and Wang, {Xiu Jie} and Zhao, {Xiao Yang} and Qi Zhou",

year = "2014",

month = mar,

day = "6",

doi = "10.1016/j.stem.2013.11.016",

language = "English (US)",

volume = "14",

pages = "404--414",

journal = "Cell stem cell",

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publisher = "Cell Press",

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TY - JOUR

T1 - Genetic modification and screening in rat using haploid embryonic stem cells

AU - Li, Wei

AU - Li, Xin

AU - Li, Tianda

AU - Jiang, Ming Gui

AU - Wan, Haifeng

AU - Luo, Guan Zheng

AU - Feng, Chunjing

AU - Cui, Xiaolong

AU - Teng, Fei

AU - Yuan, Yan

AU - Zhou, Quan

AU - Gu, Qi

AU - Shuai, Ling

AU - Sha, Jiahao

AU - Xiao, Yamei

AU - Wang, Liu

AU - Liu, Zhonghua

AU - Wang, Xiu Jie

AU - Zhao, Xiao Yang

AU - Zhou, Qi

PY - 2014/3/6

Y1 - 2014/3/6

N2 - The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of rat androgenetic haploid embryonic stem cells (RahESCs) as a tool to facilitate such studies. Our approach is based on removal of the maternal pronucleus from zygotes to generate androgenetic embryos followed by derivation of ESCs. The resulting RahESCs have 21 chromosomes, express pluripotency markers, differentiate into three germ layer cells, and contribute to the germline. Homozygous mutations can be introduced by both large-scale gene trapping and precise gene targeting via homologous recombination or the CRISPR-Cas system. RahESCs can also produce fertile rats after intracytoplasmic injection into oocytes and are therefore able to transmit genetic modifications to offspring. Overall, RahESCs represent a practical tool for functional genetic studies and production of transgenic lines in rat.

AB - The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of rat androgenetic haploid embryonic stem cells (RahESCs) as a tool to facilitate such studies. Our approach is based on removal of the maternal pronucleus from zygotes to generate androgenetic embryos followed by derivation of ESCs. The resulting RahESCs have 21 chromosomes, express pluripotency markers, differentiate into three germ layer cells, and contribute to the germline. Homozygous mutations can be introduced by both large-scale gene trapping and precise gene targeting via homologous recombination or the CRISPR-Cas system. RahESCs can also produce fertile rats after intracytoplasmic injection into oocytes and are therefore able to transmit genetic modifications to offspring. Overall, RahESCs represent a practical tool for functional genetic studies and production of transgenic lines in rat.

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SN - 1934-5909

VL - 14

SP - 404

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JO - Cell stem cell

JF - Cell stem cell

IS - 3

ER -

Genetic modification and screening in rat using haploid embryonic stem cells

Abstract

ASJC Scopus subject areas

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