TY - JOUR
T1 - Genetic variants associated with serum thyroid stimulating hormone (TSH) levels in European Americans and African Americans from the eMERGE Network
AU - Malinowski, Jennifer R.
AU - Denny, Joshua C.
AU - Bielinski, Suzette J.
AU - Basford, Melissa A.
AU - Bradford, Yuki
AU - Peissig, Peggy L.
AU - Carrell, David
AU - Crosslin, David R.
AU - Pathak, Jyotishman
AU - Rasmussen, Luke
AU - Pacheco, Jennifer
AU - Kho, Abel
AU - Newton, Katherine M.
AU - Li, Rongling
AU - Kullo, Iftikhar J.
AU - Chute, Christopher G.
AU - Chisholm, Rex L.
AU - Jarvik, Gail P.
AU - Larson, Eric B.
AU - McCarty, Catherine A.
AU - Masys, Daniel R.
AU - Roden, Dan M.
AU - De Andrade, Mariza
AU - Ritchie, Marylyn D.
AU - Crawford, Dana C.
N1 - Publisher Copyright:
© 2014, Public Library of Science. All rights reserved.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Thyroid stimulating hormone (TSH) hormone levels are normally tightly regulated within an individual; thus, relatively small variations may indicate thyroid disease. Genome-wide association studies (GWAS) have identified variants in PDE8B and FOXE1 that are associated with TSH levels. However, prior studies lacked racial/ethnic diversity, limiting the generalization of these findings to individuals of non-European ethnicities. The Electronic Medical Records and Genomics (eMERGE) Network is a collaboration across institutions with biobanks linked to electronic medical records (EMRs). The eMERGE Network uses EMR-derived phenotypes to perform GWAS in diverse populations for a variety of phenotypes. In this report, we identified serum TSH levels from 4,501 European American and 351 African American euthyroid individuals in the eMERGE Network with existing GWAS data. Tests of association were performed using linear regression and adjusted for age, sex, body mass index (BMI), and principal components, assuming an additive genetic model. Our results replicate the known association of PDE8B with serum TSH levels in European Americans (rs2046045 p=1.85 × 10-17, β=0.09). FOXE1 variants, associated with hypothyroidism, were not genome-wide significant (rs10759944: p=1.08 × 10-6, β= -0.05). No SNPs reached genome-wide significance in African Americans. However, multiple known associations with TSH levels in European ancestry were nominally significant in African Americans, including PDE8B (rs2046045 p=0.03, β= -0.09), VEGFA (rs11755845 p=0.01, β= -0.13), and NFIA (rs334699 p=1.50 × 10-3, β= -0.17). We found little evidence that SNPs previously associated with other thyroid-related disorders were associated with serum TSH levels in this study. These results support the previously reported association between PDE8B and serum TSH levels in European Americans and emphasize the need for additional genetic studies in more diverse populations.
AB - Thyroid stimulating hormone (TSH) hormone levels are normally tightly regulated within an individual; thus, relatively small variations may indicate thyroid disease. Genome-wide association studies (GWAS) have identified variants in PDE8B and FOXE1 that are associated with TSH levels. However, prior studies lacked racial/ethnic diversity, limiting the generalization of these findings to individuals of non-European ethnicities. The Electronic Medical Records and Genomics (eMERGE) Network is a collaboration across institutions with biobanks linked to electronic medical records (EMRs). The eMERGE Network uses EMR-derived phenotypes to perform GWAS in diverse populations for a variety of phenotypes. In this report, we identified serum TSH levels from 4,501 European American and 351 African American euthyroid individuals in the eMERGE Network with existing GWAS data. Tests of association were performed using linear regression and adjusted for age, sex, body mass index (BMI), and principal components, assuming an additive genetic model. Our results replicate the known association of PDE8B with serum TSH levels in European Americans (rs2046045 p=1.85 × 10-17, β=0.09). FOXE1 variants, associated with hypothyroidism, were not genome-wide significant (rs10759944: p=1.08 × 10-6, β= -0.05). No SNPs reached genome-wide significance in African Americans. However, multiple known associations with TSH levels in European ancestry were nominally significant in African Americans, including PDE8B (rs2046045 p=0.03, β= -0.09), VEGFA (rs11755845 p=0.01, β= -0.13), and NFIA (rs334699 p=1.50 × 10-3, β= -0.17). We found little evidence that SNPs previously associated with other thyroid-related disorders were associated with serum TSH levels in this study. These results support the previously reported association between PDE8B and serum TSH levels in European Americans and emphasize the need for additional genetic studies in more diverse populations.
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U2 - 10.1371/journal.pone.0111301
DO - 10.1371/journal.pone.0111301
M3 - Article
C2 - 25436638
AN - SCOPUS:84914679382
SN - 1932-6203
VL - 9
JO - PloS one
JF - PloS one
IS - 12
M1 - e111301
ER -